• Application Note

100% Aqueous Pore Dewetting Test Using Atlantis T3

100% Aqueous Pore Dewetting Test Using Atlantis T3
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates a 100% aqueous pore dewetting test using Atlantis T3 Columns.

Introduction

Compounds analysed in this application brief :

  1. Thiourea
  2. 5-Fluorocytosine
  3. Adenine
  4. Guanosine-5’-monophosphate
  5. Thymine 

Experimental

Test Conditions

Column:

4.6 x 100 mm, 3 μm

Part Number:

186003728

Mobile Phase:

10 mM NH4COOH, pH 3.0

Flow Rate:

1.3 mL/min

Injection Volume:

5.0 μL

Column Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Waters Alliance HPLC

Results and Discussion

 

% DEWET, Atlantis T3

  1. Thiourea - 12%
  2. 5-Fluorocytosine - 8%
  3. Adenine - 8%
  4. Guanosine-5’-monophosphate - 5%
  5. Thymine - 7%

% DEWET Eclipse Plus C18

  1. Thiourea - 381%
  2. 5-Fluorocytosine - 132%
  3. Adenine - 112%
  4. Guanosine-5’-monophosphate - 112%
  5. Thymine - 99%
Atlantis T3, Intermediate Coverage C18
Zorbax Eclipse Plus C18, High Coverage C18

Featured Products

WA60211, January 2009

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