This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates analysis of ephedra alkaloids.
The compounds used in this study are –
Column: |
Xterra Phenyl, 2.1 x 150 mm, 3.5 μm |
Part number: |
186001181 |
Mobile phase A: |
10 mM NH4HCO3, pH 9.5 |
Mobile phase B: |
ACN |
Flow rate: |
0.23 mL/min |
Isocratic mobile phase composition: |
90% A; 10% B |
Injection volume: |
5 μL |
Temperature: |
40 °C |
Detection: |
UV @ 254 nm |
Instrument: |
Alliance 2695, 2996 PDA |
WA20738.048, June 2002