• Application Note

Nicotinic Acid

Nicotinic Acid
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of nicotinic acid using XTerra RP18 columns.

Introduction

Nicotinic acid has been analyzed in this study.

Experimental

HPLC Conditions

Column:

XTerra RP18 4.6 x 150 mm, 5 μm (p/n: 186000492)

Mobile phase:

At pH 3.0: H2O/ACN/100 mM, NH4COOH, pH 3.0, 85:05:10

At pH 7.0: H2O/ACN/100 mM, NH4HCO3, pH 7.0, 87.5:2.5:10

At pH 10.0: H2O/ACN/100 mM, NH4HCO3, pH 10.0, 90:0:10

Flow rate:

1.0 mL/min

Injection volume:

5 μL of 250 μg/mL

Temperature:

30 °C

Detection:

UV @ 254 nm

Instrument:

Alliance 2695, 2996 PDA

Mobile Phase pH

USP Tailing

3.0

1.26

7.0

1.26

10.0

1.26

 

Results and Discussion

Featured Products

WA20738.077, June 2002

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