• Application Note

Chemical Stability Study of ACQUITY UPLC BEH Amide Columns

Chemical Stability Study of ACQUITY UPLC BEH Amide Columns
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the Chemical stability study of ACQUITY UPLC BEH Amide Columns.

Introduction

Structures

Experimental

Test Conditions

Columns:

ACQUITY UPLC BEH Amide, 2.1 x 50 mm, 1.7 μm

Part Number:

186004800

Mobile Phase A:

50/50 MeCN/H2O with 10 mM CH3COONH4, pH 5.5

Mobile Phase B:

95/5 MeCN/H2O with 10 mM CH3COONH4, pH 5.5

Flow Rate:

0.5 mL/min

Injection Volume:

2.0 μL (full loop injection mode)

Sample Concentration:

25 μg/mL each

Sample Diluent:

75/25 MeCN/MeOH

Column Temperature:

30 °C

Weak Needle Wash:

95/5 MeCN/H2O

Detection:

UV @ 254 nm

Sampling Rate:

40 pts/sec

Filter Time Constant:

0.1

Instrument:

Waters ACQUITY UPLC with ACQUITY UPLC PDA Detector

Gradient

Results and Discussion

The compounds used in this study are:

  1. Uracil
  2. 5-fluorocytosine
  3. Cytosine

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