• Application Note

Analysis of Warfarin, Dextromethorphan, Triprolidine, and Tetracaine using XBridge Shield RP18 

Analysis of Warfarin, Dextromethorphan, Triprolidine, and Tetracaine using XBridge Shield RP18 
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of warfarin, dextromethorphan, triprolidine, and tetracaine using XBridge Shield RP18 columns.

Introduction

Warfarin, an anti-coagulants old as coumadin, is used to inhibit the synthesis of clotting factors. Dextromethorphan is an antitussive drug found in many common cold and cough formulations. Triprolidine is an antihistamine that is found in common allergy formulations. Tetracaine is a local anesthetic of the ester-linkage type, related to procaine.

1. Warfarin 
2. Dextromethorphan HCl 
3. Triprolidine HCl 
4. Tetracaine 

Experimental

Test Conditions

Columns:

XBridge Shield RP18 4.6 X 50 mm, 3.5 μm p/n: 186003042

Mobile phase A:

25 mM KH2PO4, pH 7

Mobile phase B:

MeOH

Flow rate:

1.0 mL/min

Injection volume:

10 μL

Sample Concentration and Diluent:

10 μg/mL in H2O

Temp.:

30 °C

Sampling Rate:

5 points /second

Detection:

UV @ 280 nm

Time Constant:

1.0

Needle Wash:

5/95 MeOH/H2O

Instrument:

Alliance 2695 with 2996 PDA

Gradient

Time(min)

%A

%B

0.0

60

40

7.0

20

80

7.5

60

40

10.0

60

40

System Suitability Parameters

Results and Disscussion

Compounds: 1. Warfarin 2. Dextromethorphan HCl 3. Triprolidine HCl 4. Tetracaine.

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WA60203, June 2007

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