• Application Note

Endothall in Drinking Water and Soil

Endothall in Drinking Water and Soil
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights about analysis of endothall in drinking water and soil.

Introduction

The compound analyzed in this study is endothall.

Experimental

LC-MS Method

Column:

Symmetry Shield RP8, 2.1 x 100 mm, 3.5 μm

Part number:

WAT058969

Mobile phase:

5% Acetonitrile in 1% formic acid/water

Flow rate:

200 μL/min

Injection volume:

75 μL

Detection:

Electrospray (negative ion), (SIR mode, m/z = 185)

Instrument:

Waters Alliance LC-MS with Micromass Platform LC Mass Detector

GC-MS or GC-FID Method

Column:

RTX 5 capillary, 30 meters, 0.25 mm ID, 0.25 μm film thickness

Carrier gas:

Helium @ 30 cm/sec

Temp program:

40 °C initial, 8 °C/ min to 300 °C

Injection volume:

1 μL

Detection:

HP 5972 MSD, (EI, SIM mode, m/z = 123)

A) For LC-MS: No derivatization required. The MTBE* in the eluent is removed by evaporation and the extract is adjusted to a final volume of 1.0 mL with 10% methanol in water. 

B) For GC: The eluent is heated for 40 min @ 60° C to convert endothall to the dimethyl ester. The ester is then extracted with DCM**. After removal of water by treatment with Na2SO4, the DCM** extract is evaporated to a final volume of 0.5 mL. 

Soil Samples: The sample (10 g) is extracted with 35 mL pH 10 carbonate buffer (0.1M) followed by 20 mL of water. The combined extracts are adjusted to pH 2 with phosphoric acid and centrifuged. SPE is then performed using the same protocol as water samples. 

* methyl butyl ether 
** methylene chloride 

Results and Discussion

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WA31764.77, June 2003

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