• Application Note

Trimethoprim in Rat Plasma

Trimethoprim in Rat Plasma
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates the analysis of Trimethoprim in Rat Plasma using Symmetry Columns. 

Introduction

The compound analyzed in this study is Trimethoprim. 

Experimental

HPLC Method

Column:

XTerra MS C18 2.1 x 30 mm, 3.5 µm

Part number:

186000398

Mobile phase A:

1.0% NH4OH

Mobile phase B:

ACN

Isocratic mobile phase composition:

40% A; 60% B

Flow rate:

0.2 mL/min

Injection volume:

30 µL

Detection:

MS ESI+

Instrument:

Alliance 2790, Micromass Quattro Ultima

Ion source:

ESI+

Source temperature:

150 °C

Gas cell:

1.5e-3 mbar, 25 eV

Desolvation temperature:

350 °C

Cone gas flow:

150 L/hr

Drying gas flow:

600 L/hr

Cone voltage:

20V

Results and Discussion

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WA31764.154, June 2003

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