Wyatt Technology Light Scattering Instruments and Systems

MALS instruments are added to SEC, IEX, or AF4 separations for absolute determination of a protein, viral vector, or nanoparticle’s molar mass, size, conformation, titer and payload.

This three angle MALS detector couples to your HPLC to determine the absolute molar masses and sizes of macromolecules and nanoparticles in solution from 200 Da to 10 Mda (proteins) or 1 MDa (polymers). It is ideal for peptides, proteins and small viruses or polymers with a radius <50 nm.

The microDAWN MALS detector performs absolute characterization of macromolecules eluting from UPLC or UHPLC, characterizing proteins and other molecules smaller than 50 nm in radius. Its small interdetector dispersion produces minimal band broadening in order to maintain the narrow peaks typical of UHPLC.

The ultraDAWN™ real-time multi-angle light scattering (RT-MALS) instrument is designed for in-line or on-line monitoring of key attributes of macromolecules and nanoparticles such as proteins, biopolymers, gene vectors and nanodrug-delivery vehicles. It provides molar mass, size, particle concentration and payload and its data may be fed back to the process controller via OPC-UA.

This manual DLS Instrument can measure a protein, viral vector or nanoparticle’s size, polydispersity, concentration, and turbidity for aggregation, colloidal propensity and thermal stability studies.

AF4 provides low-shear, size-based separations of biologic macromolecules and nanoparticles from 1 nm to 10 µm. It works with on-line MALS, DLS, UV, RI, fluorescence, and viscometry detectors.