BioSuite Columns
Confidently isolate, analyze, and characterize proteins and peptides
Achieving excellent resolution and recovery of proteins and peptides is critical. Waters BioSuite HPLC Columns for protein and peptide analysis contain high-performance chemistries dedicated to the isolation, analysis, and protein characterization of biomolecules.
Waters BioSuite HPLC Columns support the array of Waters LC and LC-MS systems and include separation offerings such as ion-exchange, size exclusion, hydrophobic interaction, and reversed-phase columns. With BioSuite Columns for protein and peptide separations, your lab can achieve excellent resolution and recovery of proteins and peptides across different particle and pore sizes, all with the ability to scale from analytical to lab-scale preparative applications.
BioSuite Columns
Specifications
Overview
Reversed-phase columns for peptide analysis (silica-based)
Reversed-phase columns (polymethacrylate based)
Cation and anion-exchange columns (polymethacrylate based)
Size-exclusion columns (silica-based)
Hydrophilic interaction chromatography (HIC) columns (polymethacrylate based)
Recommended Use: For the separation, lab-scale isolation, and analysis of proteins or peptides.
Features Header
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Silica-based diol SEC Columns -
Molecular weight exclusion limits are determined primarily by the selected pore size
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Available in analytical and semi-preparative column sizes
Explore SEC Columns
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Specifically designed for excellent retention of polar peptides
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Excellent performance in traditional HPLC separations using low TFA concentrations (e.g., 0.025% TFA)
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Outstanding performance in separating digests containing hydrophilic, hydrophobic, and basic peptides
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Superior peak shape and capacity for peptide separations using TFA (e.g., 0.1% TFA)
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Good choice for separation of large peptide fragments (e.g., Lys-C digests)
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BioSuite HIC Columns provide a viable separation alternative to reversed-phase chromatography
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The large 1000 Å pore size accommodates proteins up to 5,000,000 daltons
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Lab-scale isolation is achievable with the 21.5 × 150 mm BioSuite Phenyl HIC Column
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Four separation chemistries including strong and weak cation (CXC) and anion exchangers (AXC) bonded to a pH stable (i.e., pH 2–12), methacrylic ester-based polymeric resin
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Provides the flexibility required to develop methods that separate proteins or peptides based upon minor charge differences
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Speed and superior chromatographic resolution achievable with the Non-Porous (NP) IEX offerings
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Porous ion exchangers are available for applications requiring greater protein or peptide binding capacity
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Selected BioSuite Ion-Exchange Columns are offered in PEEK hardware as well as in 21.5 mm I.D. preparative column sizes
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Designed to retain highly polar analytes that are too polar to retain by reversed phase
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Exceptional chemical stability at high pH and high temperature
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Ideal for sugar/carbohydrate analysis
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Exceptional retention of polar analytes spanning a wide range of polarity
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