• Application Note

Analysis of Triton X-100 using UltraPerformance Convergence Chromatography

Analysis of Triton X-100 using UltraPerformance Convergence Chromatography
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of Triton X-100 Using UltraPerformance Convergence Chromatography.

Benefits

Provides a rapid, high efficiency separation for Triton X-100.

Introduction

Non-ionic surfactants such as Triton X-100 are used in cosmetics, industrial materials, and many other products. Their composition has to be monitored because the differences in ethoxy chain length affect the viscosity, solubility, polarity, and other characteristics of the mixture.

They are typically analyzed by HPLC, SFC, and GC. Analysis by GC and HPLC can be very time-consuming, and HPLC may require derivatization for non-UV absorbing surfactants. In some cases, baseline separation for oligomers is still not achieved.

Experimental

Method Conditions

System:

ACQUITY UPC2

Detection:

Photodiode Array (PDA)

PDA 3D Channel PDA, 210 to 400 nm;

PDA 2D Channel 222 nm at 4.8 nm resolution (compensated 380 to 480 nm)

Column:

ACQUITY UPC2 BEH 2.1 x 50 mm, 1.7 μm

Mobile phase A:

CO2

Mobile phase B:

Methanol

Wash solvents:

70:30 methanol/isopropanol

Separation mode:

Gradient starting at 2% B to 35% over 1.25 min, back to 2% B in 5 s

Flow rate:

2.0 mL/min

UPC2 Manager:

1500 psi

Column temp.;

40 °C

Injection volume:

1.0 μL

Run time:

2 min

Sample:

10 mg/mL Triton X-100 in isopropanol

Software:

Empower 3

Results and Discussion

Triton X-100 standard.

Conclusion

UltraPerformance Convergence Chromatography (UPC2) provides a rapid, high efficiency separation for Triton X-100. Excellent resolution for approximately 20 oligomers is achieved in two minutes using lower temperature than in GC or traditional SFC, making UPC2 more amenable to thermally labile compounds. Compared with normal phase HPLC, there is a significant reduction in the consumption of toxic solvents.

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