Potency testing is the most frequently run assay in the cannabis and hemp industries for profiling main and emerging cannabinoids of interest. Critical for compliance with state and federal regulations, potency testing and cannabinoid profiling is also used for accurate product labeling, tracking extraction efficiency, and product formulation.
Waters potency testing and cannabinoid profiling solution is a comprehensive package that utilizes ultra performance liquid chromatography (UPLC) technology for fastest run times, maximum chromatographic resolution, and lowest solvent usage. For those who need answers fast and a rapid return on investment, the Waters Cannabinoid Analyzer Solution includes:
Webinar: Analytical Tools for Characterizing Cannabinoids for Quality, Safety, and Research Purposes
Get the flexibility of quaternary or binary solvent blending and decrease your time to result with the ACQUITY UPLC H-Class PLUS System to improve the characterization of your most complex samples.
Minimize the risk of unexpected coelutions, confirm analyte identification, and enhance the analytical value of each analysis using the ACQUITY QDa II Mass Detector.
Address routine and complex separation challenges while delivering the reliability, robustness, sensitivity, and increased throughput of convergence chromatography techniques with ACQUITY UPC2 System.
Obtain high-efficiency separations and quality data to meet your testing requirements with Arc HPLC System and easily improve the performance of your existing LC methods without compromising data quality in your analysis.
Calibration curves for 18 cannabinoids at 228 nm and individual SIR channels from 0.1–50 µg/mL.
UPC2 chromatograms showing (1) separation of the THC isomer standards, (2) analysis of CBD oil after being heated in an oven overnight at 55 °C, (3) analysis of CBD oil after being exposed to acidic conditions and heating overnight at 55 °C, and (4) analysis of untreated CBD oil (control). THC isomers that were identified in the CBD samples are indicated. The separations were achieved at 2 mL/min and 10% ethanol on the AMY1 column using 1 µL injections.
Figure A (top) - PDA chromatogram of an authentic standard mix of 8 isomeric cannabinoids at 228 nm (top) (100 mg/mL, 0.5 mL), and (beneath) Δ8-THC distillate sample A (1 mg/mL, 0.5 mL). Three components can be identified in the sample based on tR; Figure B (bottom) - PDA chromatogram at 228 nm (top) showing expanded view of UV baseline and the MS BPI (beneath).
Chromatograms from the analysis of a high Δ9-THC variety flower sample analyzed using UV at 228 nm and SIR of m/z 315 and m/z 357. MS analysis increases the confidence in the identification of Δ9-THC in the sample.
