• Application Note

Caffeine Metabolites- pH 10.0

Caffeine Metabolites- pH 10.0
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of caffeine metabolites using XTerra Phenyl Columns. 

Introduction

The compounds analyzed in this study are:

  1.  1-Methylxanthine 
  2. 1, 7-Dimethyluric acid 
  3. 1, 3-Dimethyluric acid 
  4. Impurity of 1, 7-Dimethylxathine 
  5. 1, 7-Dimethylxathine 
  6. Theobromine 
  7. Caffeine 

Experimental

Conditions

Column:

XTerra Phenyl, 4.6 x 150 mm, 5 µm

Part number:

186001146

Mobile phase A:

H2O

Mobile phase B:

ACN

Mobile phase C:

100 mM NH4HCO3, pH 10.0

Flow rate:

1.0 mL/min

Injection volume:

10 µL

Temperature:

30 °C

Detection:

UV @ 280 nm

Instrument:

Alliance 2695, 2996 PDA

Gradient Table

Results and Discussion

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WA20738.019, June 2002

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