This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights the analysis of tricyclic antidepressants using XBridge C18 Columns at pH 7.0.
Tricyclic antidepressants are analyzed in this application brief.
Column: |
XBridge C18, 5 μm, 4.6 x 150 mm p/n: 186003116 |
Isocratic Mobile Phase: |
20 mM sodium phosphate buffer, pH 7.0/acetonitrile/methanol (30:35:35) |
Flow Rate: |
1.5 mL/min |
Injection Volume: |
10 μL |
Sample Concentration: |
20 μg/mL in water |
Temperature: |
25 ˚C |
Detection: |
UV @ 254 nm |
Instrument: |
Alliance 2695 with 2996 PDA |
WA60167, April 2007