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Analysis of Morphine and Its Polar Metabolites Using Atlantis T3 Columns

Analysis of Morphine and Its Polar Metabolites Using Atlantis T3 Columns

This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of morphine and its polar metabolites using Atlantis T3 Columns.

Introduction

The compunds used in this study are -

10-Hydroxymorphine
Morphine-3ß-D-glucuronide
Morphine-6ß-D-glucuronide
Morphine
Morphine N-oxide
6-Acetylmorphine

Experimental

Test Conditions

Column:	Atlantis T3, 2.1 x 50 mm, 3 μm
Part number:	186003717
Mobile phase A:	0.1% FA in water
Mobile phase B:	ACN
Flow rate:	0.5 mL/min
Injection volume:	15 μL
Column temperature:	45 °C
MRM:	morphine 286 > 201 morphine-3ß-D-glucuronide 462 > 286 morphine-6ß-D-glucuronide 462 > 286 morphine N-oxide 302 > 162 6-acetylmorphine 328 > 165 10-hydroxymorphine 302 > 58
Instrument:	Waters ACQUITY UPLC System

Gradient

Time (min)	Profile
	%A	%B
0.00	98	2
5.00	2	98

Results and Discussion

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WA60210, January 2009