重新設想一種精簡、快速且具有高靈敏度的方法,用以分析酶解後的 N-醣。GlycoWorks RapiFluor-MS N-Glycan Kit(GlycoWorks RapiFluor-MS N-醣套組)僅需 3 個簡單的步驟,在短短 30 min 內,就能完成複雜、耗時的樣品前處理。RapiFluor-MS (RFMS) 的獨特化學特性有助於增強螢光定量,提供優異的質譜訊號。現在,只需要單一標記,您就能取得從表徵分析到例行監測的重要資訊。聚醣分析變得更容易、更靈敏。此入門套組內含 GlycoWorks Deglycosylation Module(GlycoWorks 去醣基化模組)、GlycoWorks Labeling Module(GlycoWorks 標記模組)、GlycoWorks Clean-up Module(GlycoWorks 淨化模組)、GlycoWorks Sample Collection Module(GlycoWorks 樣品收集模組)及 ACQUITY UPLC Glycan BEH Amide, 1.7 µm, 2.1 x 150 Column(ACQUITY UPLC 聚醣 BEH Amide,1.7 µm,2.1 x 150 管柱)和 Mobile-Phase Concentrate: Ammonium Formate(移動相濃縮液:甲酸銨)。此套組內含足以處理 96 個樣品的材料(4 x 24 格式)。此套組包含:
GlycoWorks Deglycosylation Module(GlycoWorks 去醣基化模組):
(4) 0.035 mL/瓶 Rapid PNGaseF Enzyme(Rapid PNGaseF 酶)
(4) 0.25 mL/瓶 GlycoWorks Rapid Buffer(GlycoWorks Rapid 緩衝液)
(1) 4 瓶(每瓶 10 mg)RapiGest SF Surfactant(RapiGest SF 界面活性劑)
(1) Intact mAb Mass Check Standard(完整 mAb 質量檢查標準品) *裝在單獨的盒子中
GlycoWorks RapiFluor-MS Labeling Module(GlycoWorks RapiFluor-MS 標記模組):
(4) 23 mg/瓶 RapiFluor-MS Reagent(RapiFluor-MS 試劑)
(4) 1 mL/瓶 Reagent Solvent(試劑溶劑,無水 DMF) GlycoWorks RFMS Clean-up Module(GlycoWorks RFMS 淨化模組):
(4) 0.5 mL/瓶 Elution Buffer(洗脫緩衝液,200 mM 醋酸銨 95/5 水/ACN pH 7)
(4) 3 mL/瓶 Sample Diluent(樣品稀釋液,DMF/ACN 混合溶液)
(1) GlycoWorks HILIC µElution Plate(GlycoWorks HILIC µElution 孔盤)
GlycoWorks Sample Collection Module(GlycoWorks 樣品收集模組):
(15) 1 mL 微量離心管,用於去醣基化和標記反應
(15) 600 µL 離心管內插管,用於收集 SPE 洗脫液
(15) 帶狹縫的連排蓋,用於直接進樣
(1) 1 mL 收集圓盤
(1) 廢液盤
其他:
(1) 5050 mM Ammonium Formate Solution-Glycan Analysis(5050 mM 甲酸銨溶液 - 聚醣分析,186007081)
(1) ACQUITY UPLC Glycan BEH Amide Column(ACQUITY UPLC 聚醣 BEH Amide 管柱,130 Å,1.7 µm,2.1 x 150 mm,186004742)
(1) RapiFluor-MS Glycan Performance Test Standard(RapiFluor-MS 聚醣效能測試標準品)
Number of Analysis |
96 |
Type |
Sample Preparation Workflow |
Packages per kit |
7 |
Separation Mode |
HILIC |
Detection Method |
HRMS, MS, MS/MS, UV |
Storage Condition |
Refer to the storage recommendations of each sub-package |
系統類型 |
LC-MS |
Shelf Life |
Refer to the storage recommendations of each sub-package |
UNSPSC |
41116104 |
應用 |
聚糖 |
品牌 |
GlycoWorks |
產品類型 |
應用套組 |
Units per Package |
1 pk |