HPLC Columns

HPLC Columns

50+ years of quality column manufacturing delivers reproducibility and transferability you can trust.

Waters offers an extensive selection of analytical and preparative columns, designed to meet diverse HPLC needs. With over 2,500 formats and 100+ configurations, you can confidently address challenging separations. Their 25+ unique column phases allow fine-tuning of methods for optimal results. Waters is a leader in HPLC, providing exceptional batch-to-batch reproducibility, ensuring reliable and repeatable analyses. Their column portfolio offers versatility and quality for various applications, from routine analytical work to demanding preparative separations in the laboratory.

XBridge Columns: BEH (Ethylene Bridged Hybrid) columns are known for their exceptional stability at both low and high pH levels. They are compatible with various mobile phase additives or buffers, facilitating faster method development. These universal columns are well-suited for a wide range of compounds, and with multiple pore diameters available, they are optimized for the separation of molecules of different sizes, providing a comprehensive selection for various analytical needs.

XSelect Columns: These columns offer a wide selectivity range with diverse base particles and ligands, making them perfect for method scouting and development. They are well-suited for analyzing all types of samples in both HPLC and LC-MS analyses. CSH columns excel in providing excellent peak shape for basic compounds at low pH, while HSS columns deliver increased retention for polar and non-polar compounds, catering to the needs of various chromatographic applications.

Atlantis Columns: These columns utilize silica particles with mid-coverage C18 bonding, providing exceptional retention for polar compounds. They offer balanced retention for diverse analyte mixtures and are compatible with 100% aqueous mobile phases. Moreover, these columns demonstrate long lifetimes even when operating with low mobile phase pH, making them ideal for a wide range of chromatographic applications, especially those involving polar compounds and harsh mobile phase conditions.

Symmetry Columns: These columns offer excellent column-to-column and batch-to-batch reproducibility, ensuring consistent and reliable results in chromatographic analyses. They utilize embedded polar group technology, which enhances peak shape for basic analytes, making them ideal for separating challenging compounds. Being the first to introduce high purity silica particles, these columns are designed to meet the highest standards of reproducibility, providing precise and repeatable chromatographic performance.

SunFire Columns: The trifunctionally bonded high purity silica particle offers enhanced stability under acidic conditions, ensuring reliable performance even in challenging environments. It allows for superior mass loading when operating at low pH levels, making it suitable for high-throughput analyses. Additionally, this column demonstrates excellent performance and durability when used with trifluoroacetic acid (TFA), providing long-lasting and efficient chromatographic results.

HPLC Columns FAQs

1.What is an HPLC column, and how does it work?
An HPLC (High-Performance Liquid Chromatography) column is a tube packed with a stationary phase material that separates compounds in a liquid sample as it passes through. The separation occurs based on the interactions between the compounds, the stationary phase, and the mobile phase (the liquid carrying the sample). Different compounds exit the column at different times, allowing for their identification and quantification.

2.What types of HPLC columns does Waters offer?
Waters offers a wide range of HPLC columns tailored for various applications, including reversed-phase, normal-phase, size exclusion, and ion exchange chromatography. These columns are designed to handle different sample types, from small molecules to large biomolecules like proteins.
For example, XBridge columns are designed for robust and reliable separations, offering excellent stability across a wide pH range, making them ideal for challenging analytical applications. SunFire columns provide high efficiency and peak symmetry for a wide variety of compounds, ensuring consistent and reproducible results in both routine and complex analyses. These are just two types of HPLC columns that Waters offers.

3.What is the difference between reversed-phase and normal-phase HPLC columns?
Reversed-phase HPLC columns use a non-polar stationary phase and a polar mobile phase, making them ideal for separating non-polar compounds. Normal-phase HPLC columns, on the other hand, use a polar stationary phase and a non-polar mobile phase, which is suited for separating polar compounds. The choice between these columns depends on the nature of the sample and the separation requirements in your laboratory.

4. Can I reuse an HPLC column, and how do I know when it needs to be replaced?
Yes, HPLC columns can often be reused, but their lifespan depends on the sample type and usage conditions. Signs that a column needs replacement include increased backpressure, loss of resolution, or changes in retention times. Regular maintenance, such as proper cleaning and storage, can extend a column"s life, but eventually, all columns will degrade and require replacement to maintain accurate results.