Size Exclusion Chromatography (SEC) 

Size Exclusion Chromatography (SEC) 

Size-based separations of biological compounds start here 

Size-based separations of biological compounds start here 

Size-exclusion chromatography (SEC) is a powerful technique for aggregate and fragment analysis in the research, development, and manufacturing of biotherapeutic proteins, such as insulin and monoclonal antibodies (mAb). 

Get unmatched batch-to-batch reproducibility with the wide range of Waters SEC columns and standards for peptides and proteins characterization. 

LC system dispersion can significantly affect SEC column choice.

Find the right SEC column for your lab with the system dispersion specifications for column recommendations below. 

LC system dispersion can significantly affect SEC column choice.

Find the right SEC column for your lab with the system dispersion specifications for column recommendations below. 

LC system dispersions <20 μL
(UPLC)

1.7 μm column

LC system dispersions >20 μL - <35 μL
(UHPLC)

2.5 μm column

LC system dispersions >35 μL - <35 μL
(HPLC)

2.5 μm or 3.5 μm column

Columns

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  • 2.5 μm particle columns for UPLC, UHPLC and HPLC separations
  • Pore size options include: 200 Å
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  • Sub-2-μm particle columns for UPLC separations
  • Pore size options include: 125 Å, 200 Å, and 450 Å
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  • 2.5 μm particle columns for HPLC and UHPLC separations
  • Pore size options include: 125 Å, 200 Å, and 450 Å

All Waters SEC Columns are:

All Waters SEC Columns are:
  • Manufactured in a cGMP, ISO 9001 certified plant using stringent manufacturing protocols and ultra-pure reagents.
  • Quality control tested with relevant samples to ensure consistency
  • Consistent, with reliable column-to-column and batch-to-batch performance
  • Built with Ethylene Bridged Hybrid (BEH) particle technology
  • Ideal for method flexibility with pH stable >pH 8
  • Designed with a stable diol-coating for less ionic interaction, enhanced sample recovery, and improved separation reproducibility

Standards

BEH SEC Protein Standards

BEH SEC Protein Standards
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Ensure column and system performance with the BEH SEC Protein Standard, designed to benchmark ACQUITY and XBridge SEC columns.

mAb Size Variant Standard

mAb Size Variant Standard
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Ensure system performance and reliability in separating mAb aggregates, monomer, and fragments / clips via SEC with this specifically designed NIST-based standard for BioResolve SEC mAb Columns.

Product Reviews

Product Reviews
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