• Application Note

Enkephalins

Enkephalins
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief demonstrates analysis of enkephalins.

Introduction

The peptides used in this study are –

Peptides

USP Tailing Factors

Met enkephalin: Tyr-Gly-Phe-Met

0.99

Leu enkephalin: Tyr-Gly-Gly-Phe-Leu

0.97

Experimental

HPLC Method

Column:

Symmetry C8, 3.9 x 150 mm, 5 μm

Guard column:

Sentry Guard Column 3.9 x 20 mm, 5 μm

Part numbers:

Column - WAT046970, Guard - WAT054250

Mobile phase A:

0.1% TFA

Mobile phase B:

0.1% TFA in acetonitrile/water 90:10

Flow rate:

1.0 mL/min

Injection volume:

5 μL of mixture with 500 μg/mL of each peptide

Detection:

UV @ 214 nm

Gradient

Results and Discussion

Featured Products

WA31763.82, June 2003

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