This is an Application Brief and does not contain a detailed Experimental section.
This application brief highlights the analysis of morphine and its glucuronide metabolites using Oasis HLB extraction method.
Compounds studied in this application brief are:
1. Morphine-D3-glucuronide
2. Morphine-D6-glucuronide
3. Morphine
Column: |
SymmetryShield RP8, 3.9 x 150 mm, 5 μm (p/n: WAT200655) |
Mobile phase: |
20 mM potassium phosphate, pH 6.4 |
Flow rate: |
1.0 mL/min |
Injection volume: |
100 μL of porcine serum extract |
Detection: |
Fluorescence; ex 280 nm, em 355 nm |
WA31764.100, June 2003