• Application Note

Analysis of Catechins Using XBridge Phenyl

Analysis of Catechins Using XBridge Phenyl
  • Waters Corporation
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This is an Application Brief and does not contain a detailed Experimental section.

Abstract

This application brief highlights the analysis of catechins using XBridge Phenyl columns.

Introduction

Experimental

Test Conditions

Columns:

XBridge Phenyl, 4.6 x 150 mm, 3.5 μm p/n: 186003335

Mobile phase A:

H2O

Mobile phase B:

MeOH

Mobile phase C:

0.2% HCOOH in H2O

Flow rate:

0.8 mL/min

Injection volume:

20 μL

Sample:

Gallic Acid (10 μg/mL), Epigallocatechin (50 μg/mL),

Catechin (50 μg/mL),

Caffeine (10 μg/mL),

Epigallocatechin Gallate (50 μg/mL),

Gallocatechin Gallate (50 μg/mL), Epicatechin Gallate (10 μg/mL), Catechin Gallate (50 μg/mL) in H2O/MeOH (85/15)

Column temp.:

30 °C

Sampling temp.:

15 °C

Detection:

UV @ 280 nm

Sampling Rate:

5 points/sec

Filter Response:

0.2

Instrument:

Alliance 2695 with 2996 PDA

Gradient

Time(min)

%A

%B

%C

0.00

84

15

1

8.00

84

15

1

15.00

62

37

1

20.00

62

37

1

26.00

84

15

1

30.00

84

15

1

Results and Discussion

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WA60190, June 2007

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