This is an Application Brief and does not contain a detailed Experimental section.
This application brief demonstrates the UPLC Analysis of water soluble vitamins.
1. Nicotinamide (25 μg/mL)
2. Pyridoxal (50 μg/mL)
3. Riboflavin (50 μg/mL)
4. Nicotinic acid (50 μg/mL)
5. Thiamine (50 μg/mL)
6. Ascorbic acid (25 μg/mL)
7. B12 (50 μg/mL)
8. Folic Acid (25 μg/mL)
Column: |
ACQUITY UPLC BEH Amide, 2.1 x 50 mm, 1.7 μm |
Part Number: |
186004800 |
Mobile Phase A: |
50/50 MeCN/H2O with 10 mM CH3COONH4 and 0.04 % NH4OH, pH 9.0 |
Mobile Phase B: |
90/10 MeCN/H2O with 10 mM CH3COONH4 and 0.04 % NH4OH, pH 9.0 |
Flow Rate: |
0.5 mL/min |
Injection Volume: |
5 μL (PLNO) |
Sample Diluent: |
75/25 MeCN/MeOH with 0.2% HCOOH |
Column Temperature: |
30 °C |
Weak Needle Wash: |
95/5 MeCN/H2O |
Detection: |
UV @ 265nm |
Sampling Rate: |
20 points/sec |
Filter Time Constant: |
0.2 |
Instrument: |
Waters ACQUITY UPLC with ACQUITY UPLC PDA Detector |
Time (min) |
%A |
%B |
---|---|---|
Initial |
0.1 |
99.9 |
3.50 |
70.0 |
30.0 |
3.51 |
0.1 |
99.9 |
7.50 |
0.1 |
99.9 |
WA60093, June 2009