Atlantis Premier Columns

Atlantis Premier Columns

Next-generation hybrid particle and surface technology for increased polar analyte retention

Next-generation hybrid particle and surface technology for increased polar analyte retention

Retaining polar compounds is one of the most challenging chromatographic problems. With the hybrid particle technology in Atlantis Premier BEH C18 AX and the BEH Z-HILIC Columns, those problems are a thing of the past. These reversed-phase, anion-exchanged, mixed-mode, and zwitterionic columns are the next evolution of columns in the Atlantis family, all designed to achieve superior polar compound retention.

Atlantis Premier BEH C18 AX and the BEH Z-HILIC Columns combine a new and innovative chromatographic particle technology with proprietary MaxPeak High-Performance Surfaces (HPS) Technology to boost retention of polar acidic compounds by 50% while eliminating unwanted analyte-surface interactions.

Atlantis Premier Column with VanGuard FIT Atlantis Premier Columns


Waters offers a wide selection of Atlantis Column chemistries.

Waters offers a wide selection of Atlantis Column chemistries.

Specifications

Overview

  • Achieves 50% more retention than existing BEH columns with its high-retention BEH 95 Å particle
  • Compatible with 100% aqueous mobile phases
  • Increase selectivity, method flexibility, and acid retention over a wider pH range (2–10) with mixed mode, reversed-phase, anion exchange chemistry
  • Reduces analyte surface interactions with MaxPeak High Performance Surfaces (HPS)
  • Longer column lifetimes with expected column performance

Recommended Use: For improved retention of polar neutral and acidic compounds under reversed-phase conditions.


Atlantis Premier Column Featured Technologies

Atlantis Premier Column Featured Technologies

MaxPeak HPS Technology
  • Reduce analyte/surface interactions
  • Increase analyte recovery
  • Better peak shape
  • Increase sensitivity
VanGuard FIT*
  • All-in-one guard, plus column design
  • Achieve longer column lifetimes
  • Easy installation

* Specific to columns noted with VanGuard FIT

Atlantis Premier Column Characteristics

Atlantis Premier Column Characteristics

Atlantis Premier BEH 95 Å Particle Columns
  • Mixed-mode C18 Anion-exchange (AX) Chemistry
  • Increase retention of polar acids
  • Wider pH range (2–10)
  • Batch-to-batch reproducibility
  • Mass Spectrometry (MS) compatible
Atlantis Premier BEH Z-HILIC Columns
  • Uses sulfobetaine zwitterionic ligand
  • Increase retention of strongly polar analytes
  • Wider pH range (2–10)
  • Batch-to-batch reproducibility
  • Mass Spectrometry (MS) compatible

Superior batch-to-batch reproducibility

The surface modification of Waters Atlantis Premier BEH C18 AX Columns involves separate steps to incorporate the charge modifier, the C18 groups and the endcap. This approach provides excellent control of the surface chemistry, resulting in high batch-to-batch reproducibility.


Extended pH stability for method flexibility

Combining the base-stable BEH particle with stable bonding chemistries, the Waters Atlantis Premier BEH C18 AX Column material shows exceptional resistance to both acidic and basic mobile phases for a mixed-mode stationary phase. The extended high pH stability of Atlantis Premier BEH C18 AX Columns enables labs to use a wider range of mobile phase pH values.


Selectivity that complements traditional reversed-phase

The selectivity of the Waters Atlantis Premier BEH C18 AX stationary phase is very different from traditional C18 bonded phases like HSS T3 or the C18-bonded charged surface hybrid, or CSH. The BEH C18 AX material provides the greatest retention of the acidic compound and the least retention for the base procainamide.



Resources

Documents

Documents


Support

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Related

Improve chromatographic performance, retention, stability, and method development versatility for polar compounds with Atlantis HPLC Columns.

Experience the highest level of chromatographic performance, flexibility, and assurance while mitigating unwanted analyte/surface interactions with MaxPeak Premier Columns.

Prolong analytical column lifetime while maintaining chromatographic performance with VanGuard Pre-columns and cartridges.

Experience the ultimate in sensitivity, reproducibility, and speed. The ACQUITY Premier LC-MS provides the best sensitivity and peak shape for analytical separations.

Offers and Discounts

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Atlantis Premier Columns are a new and advanced tool used in liquid chromatography, which is a technique used in laboratories to separate and analyze different compounds in a mixture.

These columns have several advantages that make them really helpful for scientists. One important feature is that they improve the shape of the peaks in the chromatogram. In chromatography, the peaks represent different compounds, and having well-shaped peaks makes it easier to see and measure them accurately. If the peaks are messy or overlap, it can be hard to tell them apart and get accurate results. Atlantis Premier Columns use special materials and designs to make the peaks sharper and more symmetrical, which helps scientists get better results.

Another great thing about these columns is that they reduce unwanted interactions between the sample compounds and the column material. Sometimes, the compounds in a sample can stick to the column in a way that affects the separation and makes the peaks distorted or less clear. Atlantis Premier Columns are designed to minimize these interactions, which leads to cleaner results and better sensitivity, especially when dealing with complex samples.

These columns are also very reliable and can be used for many analyses without losing their effectiveness. This is really important for labs where consistent results are crucial for their work.

In short, Atlantis Premier Columns are a big step forward in liquid chromatography technology. They help improve peak shape, reduce unwanted interactions, and provide consistent and reliable results, making them valuable tools for scientists and researchers.

Atlantis Premier Columns FAQs

1. What types of separations are Atlantis Premier Columns best for?
Atlantis Premier Columns are designed for top-tier performance across a variety of challenging separations, making them highly versatile for specialized applications. They excel in biomolecule separations, effectively isolating peptides, proteins, and other biomolecules with outstanding resolution, sensitivity, and reproducibility. Additionally, these columns are well-suited for chiral separations, providing efficient enantiomer resolution essential for many analytical applications. In hydrophilic interaction chromatography (HILIC), Atlantis Premier Columns are particularly effective at retaining polar compounds like sugars, nucleosides, and metabolites, which are typically challenging to separate on reversed-phase columns. These columns also support mixed-mode separations, which combine reversed-phase and hydrophilic interaction mechanisms to achieve unique selectivity across complex sample types.

2. What is the difference between Atlantis Premier and traditional Atlantis columns?
Atlantis Premier Columns mark a significant advancement over traditional Atlantis columns, offering enhanced performance, durability, and versatility. One of the primary distinctions is their improved peak shape, resolution, and sensitivity, which lead to more precise and reliable analytical results. These columns are also more durable, with increased resistance to column damage, extending their lifespan and reducing maintenance needs. In addition, they operate over a wider pH range, accommodating a broader range of compound separations. Another advantage is their improved reproducibility, ensuring consistent results between batches and enhancing overall data quality, which is particularly valuable in method development and routine analysis.

3. What is the typical lifetime of an Atlantis Premier Column?
The typical lifespan of an Atlantis Premier Column varies depending on factors like sample type, mobile phase composition, flow rate, and usage frequency, but with proper care, these columns can last several months to years. To extend column life, it’s important to avoid running the column dry, which can damage the stationary phase. Use mobile phases compatible with the column to prevent degradation, and always filter the mobile phase to avoid particulate clogging. Proper storage in a clean, dry environment at room temperature is essential, along with regular cleaning to eliminate contaminants.