XSelect Premier Columns

XSelect Premier Columns

Maximize selectivity with problem-solving technologies for a wide range of analytes

Maximize selectivity with problem-solving technologies for a wide range of analytes

Get the best injection to injection reproducibility in your chromatographic separations with Waters XSelect Premier 2.5 µm and 3.5 µm Columns, which utilize MaxPeak High Performance Surfaces (HPS) Technology. Ideal for the wide range of analytes encountered in method development, XSelect Premier Columns combine modern particle technologies and ligands with the protection of MaxPeak HPS Technology. 

Designed to maximize selectivity, XSelect Premier Columns are built on one of two innovative base particle technologies, Charged Surface Hybrid (CSH) and High Strength Silica (HSS). The integration of MaxPeak HPS allows you to recover more and achieve balanced, controlled interactions between your analytes, mobile phase, and the column for the highest performance chromatographic separations.

XBridge Premier UHPLC Columns are designed to operate under the extremes of temperature and mobile phase pH while providing improved analyte peak shape by mitigating unwanted analyte/surface interactions.  XSelect Premier Columns
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Waters offers a wide selection of XSelect Column chemistries and are now available in Optimum Bed Density (OBD) Preparative Columns.

Waters offers a wide selection of XSelect Column chemistries and are now available in Optimum Bed Density (OBD) Preparative Columns.

Specifications

Overview

  • Reduces column conditioning and passivation for higher productivity
  • Maximizes selectivity with excellent stability at low pH
  • Improves sensitivity and peak shape for basic compounds
  • Increases reproducibility from analysis to analysis, for more confidence in analytical results
  • Prolongs column lifetimes with optional VanGuard FIT format 

Recommended Use: For improving selectivity, peak shape, and retentivity in method development.

XSelect Premier Column Featured Technologies

XSelect Premier Column Featured Technologies
MaxPeak HPS Technology
  • Reduce analyte/surface interactions
  • Increase analyte recovery
  • Better peak shape
  • Increase sensitivity
  • Improved peak purity
VanGuard FIT*
  • All-in-one guard, plus column design
  • Achieve longer column lifetimes
  • Easy installation

* Specific to columns noted with VanGuard FIT

XSelect Premier CSH Column Characteristics

XSelect Premier CSH Column Characteristics
XSelect Premier Peptide CSH C18 Columns
  • Alternative selectivity to existing C18 columns
  • Low anion exchange
  • General purpose hybrid columns
  • Improved peak shape for bases with low pH mobile phase
  • Reduction in analyte/surface interactions
  • Excellent pH stability
  • VanGuard FIT column option
XSelect Premier CSH Phenyl-Hexyl Columns
  • Alternative selectivity for aromatic compounds
  • Medium anion exchange
  • General purpose hybrid columns
  • Improved peak shape for bases with low pH mobile phase
  • Reduction in analyte/surface interactions
  • Excellent pH stability
  • VanGuard FIT column option

XSelect Premier HSS Column Characteristics

XSelect Premier HSS Column Characteristics
XSelect Premier HSS T3 Columns
  • Excellent reversed-phase retention for polar analytes
  • 100% water wettable
  • General purpose silica columns
  • High retention
  • Reduction in analyte/surface interactions
  • Excellent low pH stability
  • VanGuard FIT column option

XSelect Premier HPLC and UHPLC Application-Specific Column Characteristics

XSelect Premier HPLC and UHPLC Application-Specific Column Characteristics
XSelect Premier Peptide CSH C18 Columns
  • Outstanding peak capacities with formic acid for MS-based applications
  • Excellent performance with TFA for optical based applications
  • Less dependence on TFA or FA to achieve optimal peak capacity
  • Accepts greater peptide mass loads than many competitive technologies for detection of low-level impurities
  • At low pH with the positive charge on CSH, you get less secondary interactions with the particle
  • Superior peak shape and reduced tailing for acidic peptides
  • Enhanced recovery and sensitivity for acidic peptides
  • Available in OBD preparative 3.5 and 5 µm particles for seamless scalability and enhanced peptide isolation and purification

Try  XSelect Premier Peptide CSH OBD Preparative Columns

XSelect Premier Peptide HSS T3 Columns
  • Separations that require silica-based selectivity
  • Separations that necessitate increased peptide retention
  • Ideally suited for small, hydrophilic, polar peptides
  • Superior peak shape and reduced tailing for acidic peptides
  • Enhanced recovery and sensitivity for acidic peptides

Try XSelect Premier Peptide HSS T3 Columns

The selectivity you need for method development

Maximize selectivity while minimizing variability with Waters XSelect Premier Columns, which provide the chemistries to enhance selectivity for method development while preventing unwanted analyte-surface interactions that can cause variability.

Two base particles, HSS and CSH, provide enhanced selectivity for acidic, basic, and neutral compounds across a wide pH range. For formic acid mobile phases, XSelect Premier CSH C18 Columns are the first choice for method development. You can enhance selectivity for aromatic compounds with XSelect Premier Phenyl-Hexyl Columns. Finally, increase retention for polar compounds under reversed-phase LC conditions with XSelect Premier HSS T3 Columns, which are compatible with 100% aqueous mobile phases.

The results you want. None of the interactions you don’t

When developing analytical methods, having the confidence that analytes will not interact with metal ions in the flow path is critical. Analyte/surface interactions can lead to poor peak shape, analyte loss/misidentification, and ultimately costly sample re-work. The MaxPeak HPS Technology found in Waters XSelect Premier Columns significantly reduces unwanted analyte/surface interactions, so you can be sure you’re seeing everything in your sample --from the first injection on.

Stay compliant with smart column options

Ideal for compliant laboratories, smart column options are available for identifying and tracking column information. The eCord is a data recording device which is permanently attached to the column body of 2.x μm particle size (2.1 and 3.0 mm internal diameter) and ACQUITY UPLC columns and is standard for those column offerings. Rather, the eConnect column tag is a permanently attached electronic tag device for HPLC columns (≥ 2.x μm particles) used with Alliance iS and Alliance iS Bio HPLC Systems that are optional. Data from column devices (eCord device or eConnect column tag) can be accessed using the respective system’s console. These smart-column abilities provide a quick overview of column history and can be utilized as a basic troubleshooting tool. Data residing on these devices are automatically imported by and later searchable via Empower CDS.

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Related

XSelect Columns
Get superior selectivity and peak shape for basic compounds with XSelect HPLC and UHPLC columns. Discover our CSH and high-strength silica columns.

MaxPeak Premier Columns
Experience the highest level of chromatographic performance, flexibility, and assurance while mitigating unwanted analyte/surface interactions with MaxPeak Premier Columns.

Arc Premier System
Confidently produce the most accurate, reliable, and reproducible data. The Arc Premier System provides right first time robust results from the very first injection, reliably and easily.

VanGuard Column Protection
Prolong analytical column lifetime while maintaining chromatographic performance with VanGuard Pre-columns and cartridges.

Offers and Discounts

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XSelect Premier UHPLC Columns, made by Waters Corporation, are a big step forward in chromatography. They're carefully designed and built to work really well for lots of different separation tasks.

The "XSelect" label encompasses a range of columns tailored to meet diverse chromatographic needs, while "Premier" denotes their top-tier status within this series. Utilizing cutting-edge technologies and premium materials, these columns are intricately engineered to ensure superior chromatographic results.

What distinguishes XSelect Premier Columns is their consistent ability to achieve high-resolution separations, even with complex samples. This capability is particularly valuable across multiple analytical sectors, including pharmaceuticals, environmental analysis, and the food and beverage industry.

Crafted with precision-packed stationary phases carefully selected to enhance interactions with target analytes, these columns guarantee high selectivity, resolution, and efficiency. This meticulous approach enables researchers to obtain precise and dependable results with confidence.

Moreover, XSelect Premier Columns are renowned for their durability and reproducibility, essential qualities in analytical workflows. Researchers can rely on these columns to deliver consistent results over time, minimizing fluctuations and maintaining data integrity.

In addition to their exceptional performance, XSelect Premier Columns prioritize user convenience. They are compatible with a wide range of chromatography systems, making them versatile and adaptable to various laboratory setups and procedures.

In conclusion, XSelect Premier Columns represent the epitome of chromatographic excellence, offering researchers unmatched performance, reliability, and flexibility for their analytical separations.

XSelect Premier Columns FAQs

What applications are XSelect Premier Columns best suited for?
XSelect Premier Columns are ideal for applications requiring high-resolution separations, robust performance, and improved analyte recovery. They are particularly well-suited for pharmaceutical and biopharmaceutical analyses, including small molecule separations, impurity profiling, and stability testing. These columns also excel in complex sample matrices, making them valuable for environmental, food safety, and clinical research applications. Their ability to reduce secondary interactions and enhance peak shape makes them an excellent choice for method development and analyte characterization across a broad range of industries.

What stationary phase options are available for XSelect Premier Columns?
XSelect Premier Columns are available with a variety of stationary phases to meet diverse separation needs. These include reversed-phase chemistries such as C18, C8, phenyl, and polar-embedded options, as well as HILIC (hydrophilic interaction chromatography) phases for polar analytes. Each stationary phase is designed to offer unique selectivity and separation performance, enabling users to tailor their methods for specific compounds or mixtures.

Are XSelect Premier Columns compatible with both HPLC and UPLC systems?
Yes, XSelect Premier Columns are designed for compatibility with both HPLC and UPLC systems, providing flexibility for users transitioning between platforms. They are available in a range of particle sizes, including sub-2-µm particles for UPLC and larger particles for HPLC. This dual compatibility allows users to take advantage of the columns" advanced separation capabilities regardless of the system, making them suitable for both high-efficiency workflows and legacy instrumentation.

What is the pressure limit for these columns?
The pressure limit for XSelect Premier Columns depends on the particle size and system compatibility. For sub-2-µm particle columns used with UPLC systems, the maximum operating pressure typically ranges up to 15,000 psi (1,034 bar). For columns designed for HPLC with larger particles, the pressure limit is generally lower, around 6,000 psi (414 bar). It is important to consult the column specifications for exact pressure ratings and ensure that your system operates within these limits to maintain optimal performance and column integrity.