Amino Acid Analysis Standards & Kits

Amino Acid Analysis Standards & Kits

Accurate amino acid composition analysis for HPLC, UHPLC, and UPLC

Accurate amino acid composition analysis for HPLC, UHPLC, and UPLC

Accurately separating, identifying, and quantitating amino acids is challenging. The amino acid sequence is the primary structure of a protein and it is unique to a particular peptide or protein. Amino acids are also key ingredients in the cell culture media and feed used to prepare recombinant proteins. Determining amino acid composition in protein/peptide hydrolysates, foods, feeds, and pharmaceutical preparations is important, as regularly monitoring individual amino acid consumption can help you optimize the conditions in the bioreactor.

You can easily analyze amino acids with the many manual and automated solutions that use pre-column derivatization from Waters. With Waters AccQ•Tag Ultra C18, AccQ•Tag Ultra C18 – Automation, and AccQ•Tag Sample Preparation, you’ll have what your lab needs for accurate amino acid composition analysis.

AccQ•Tag Ultra and AccQ•Tag chemistries are, the first step in enabling your laboratory to obtain accurate amino acid composition from protein/peptide hydrolysates, physiologic fluids, feeds, foods, pharmaceutical preparations, and many other sample matrices. Amino Acid Analysis Standards & Kits
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Specifications

Amino Acid Standards

Amino Acid Standards

Amino Acid Kits

Amino Acid Kits

Overview

  • Achieve stable derivatives with simple, fast, and sensitive pre-column derivatization for primary and secondary amines
  • Derivatized amino acids resolved and quantified with UV, Fluorescence, and Mass Spec detection
  • Complete workflow solution including eluents, standards, derivatization reagents, columns, and methods 
  • Automation enabled on multiple platforms with verified scripts 
  • Application-specific amino acid standard kits for identification and quantification 

  • View useful sample preparation workflow guidelines for the hydrolysis and analysis of amino acids from purified peptides/proteins, foods, and feeds in the Comprehensive Guide to Hydrolysis and Analysis of Amino Acids

 

Recommended Use: For accurately separating, identifying, and quantitating amino acids.

Amino Acid Analysis Manual Kits

Amino Acid Analysis Manual Kits
AccQ•Tag Ultra and AccQ•Tag Chemistry Kits
  • Pre-column derivatization reagents
  • Dedicated reversed-phase columns quality control (QC) tested for amino acid analysis
  • Eluents and standards
  • Instrument methods for the analysis of hydrolysates, cell culture, and foods and feeds

Enable your laboratory to obtain accurate amino acid composition from protein/peptide hydrolysates, feeds, foods, pharmaceutical preparations, and many other sample matrices. Combined with HPLC, UHPLC, or UPLC, you can now have a system that is customized to separate amino acids from protein hydrolysates, cell culture media, and food/feed samples to get enhanced resolution, resulting in reliability with precise qualitative and quantitative results.

Amino Acid Analysis Automation Kits

Amino Acid Analysis Automation Kits
AccQ•Tag Ultra Derivatization Automation Kit
  • High-throughput sample prep (up to 96 samples at a time)
  • Minimal user intervention
  • Larger reagent volume to accommodate automation needs
  • Starter kits include reagents, column, eluents, application standards, and labware
  • Scripts ready for multiple automated platforms: Tecan, Hamilton, and Andrew Alliance

Simplified tools to enhance high throughput amino acid automation via an easy-to-use, automation friendly format that maximizes workflow and accommodates residual volume. Compatible with Tecan, Hamilton, and Andrew Alliance systems, the AccQ•Tag Ultra Derivatization Automation Kit provides fast, accurate, and precise amino acid analyses, translating into cost and time savings for your lab. 

Amino Acid Analysis Reference Standards

Amino Acid Analysis Reference Standards
Amino Acid Reference Standards
  • Cell Culture Standard Kit
  • Food & Feed Standard Kit
  • Amino Acid Hydrolysates Standard
  • Internal Standard (Nva)

Waters offers reference standards for Amino Acid Analysis (AAA) required in many applications in pharmaceutical and food and feed industries. A variety of standards containing free amino acids are offered for qualitative and quantitative determination of amino acids, method development, and troubleshooting of the AccQ•Tag Ultra and AccQ•Tag methods.

Amino Acid Analysis Solutions for UPLC

Amino Acid Analysis Solutions for UPLC
AccQ•Tag Ultra and AccQ•Tag Derivatization Chemistry
  • A comprehensive, fully tested set of reagents, columns, eluents, and methods
  • AccQ•Tag is optimized for use with HPLC
  • AccQ•Tag Ultra provides high-resolution separations and is optimized for use with the UPLC/UHPLC Amino Acid Analysis Application Solution

Waters AccQ•Tag technologies enable your laboratory to obtain accurate amino acid composition from protein/peptide hydrolysates, physiologic fluids, feeds, foods, pharmaceutical preparations, and myriad other samples using traditional HPLC or UHPLC instrumentation.

Fast, accurate, quantitative amino acid analysis for protein characterization and cell culture monitoring

Whether you need to determine amino acid composition of a product and compare it to the gene sequence or natural counterpart, or if you are downstream and monitoring cell culture media for critical nutrients to optimize expression efficiencies and make timely product harvesting decisions, the UPLC amino acid analysis solutions provide rugged and reliable performance to help make accurate decisions about the product throughout an organization.

Robust solution for amino acid analysis in food and feeds

Animal feeds pose several significant challenges for accurate amino acid analysis. Quantitation of amino acids released by hydrolysis is one important measure of nutritional value. A free amino acid profile can identify the origin of a food product, and in that way, detect adulteration. Free amino acids are also metabolic indicators that can be used to monitor and optimize processes such as fermentation. With Waters amino acid analysis solution, you can have confidence that products meet the manufacturing specifications and avoid costly reformulations.

Let Andrew+ pipette for you

One of the largest pain points in sample preparation is repetitive pipetting. Minimizing sources of contamination is also critical. The Andrew+ robotic pipetting platform helps alleviate those pain points and provides confidence and reliability when doing derivatization. With Andrew+ you get flexibility in the number of samples run using AccQ•Tag Ultra Automation Sample Preparation Kit (32, 64, 96 sample), verified protocols available in OneLab, compatibility with additional connected devices, self-guided installation, online troubleshooting, and help.

List of Amino Acids in Each Amino Acid Standard

Amino acid analysis is required in many applications in pharmaceutical and food and feed industries. A variety of standards containing free amino acids are offered for qualitative and quantitative determination of amino acids, method development and troubleshooting of the AccQ•Tag Ultra method.

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Andrew+ Pipetting Robot
Discover superior, repeatable, traceable pipetting with Andrew+, a fully automated pipetting robot.

Amino Acid Analysis Solutions
Waters offers three distinctively reliable methods for accurate amino acid analyses. All three utilize pre-column derivatization methods followed by reversed-phase chromatography with on-line detection of well resolved peaks using either UV absorbance or fluorescence detection.

Amino Acids Testing
Ensure product nutrition labeling compliance and adhere to food manufacturing specifications with Waters amino acid analysis solutions for accurate, simplified testing of food, beverage and feed samples.

Amino acids Testing in Food
Confirm products meet specifications and nutritional label claims with Waters manual and automated solutions for amino acid testing, enabling laboratories.

AccQ•Tag Ultra, AccQ•Tag and Pico•Tag Columns
Enable your laboratory to obtain accurate amino acid composition from protein/peptide hydrolysates, physiologic fluids, feeds, foods, pharmaceutical preparations, and myriad other samples using traditional HPLC or UHPLC instrumentation.

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A Guide to PFAS Analysis

PFAS analysis typically encompasses a range of laboratory procedures and specialized equipment utilized by environmental scientists, chemists, and researchers. These techniques are employed to identify and quantify potentially harmful PFAS substances in various environmental samples, including water, soil, and biological tissues.

Key methodologies employed in PFAS analysis encompass:

Liquid Chromatography-Mass Spectrometry (LC-MS) LC-MS stands as a robust analytical approach renowned for its ability to separate and pinpoint compounds within mixtures. It finds extensive application in PFAS analysis due to its exceptional sensitivity and specificity.

Gas Chromatography-Mass Spectrometry (GC-MS) In certain instances, GC-MS is chosen for PFAS analysis, particularly for volatile compounds.

High-Performance Liquid Chromatography (HPLC) HPLC can be effectively coupled with various detectors, such as UV and fluorescence detectors, to scrutinize PFAS compounds.

Solid-Phase Extraction (SPE) SPE represents a preparatory technique instrumental in concentrating and purifying PFAS from environmental samples prior to analysis.

Enzyme-Linked Immunosorbent Assay (ELISA) ELISA is an immunological method employed to screen for the presence of specific PFAS compounds.

Polymerase Chain Reaction (PCR) PCR-based methods serve the purpose of genetic analysis of microorganisms involved in the environmental degradation of PFAS.

It addresses the critical issue of low background contamination, crucial for precise PFAS analysis in light of their environmental significance. This product serves as a quality-control pre-screening test for 32 PFAS compounds, guaranteeing reliability in environmental sample analysis. Characterized by its mixed-mode, water-wettable polymer composition, it proves versatile in capturing a wide spectrum of PFAS compounds in complex matrices, including drinking water, ground water, soil, and food. Renowned for delivering unmatched purity, consistency, and quality, it's tailored for environmental testing laboratories and enables low-level quantification, aligning it with the growing concern of PFAS contamination in various environmental contexts.

Amino Acid Analysis Standards & Kits FAQs

1. What types of amino acid analyses can these kits support?
Waters Amino Acid Analysis Standards & Kits are designed to support a wide range of analyses, including the quantification of free amino acids, profiling of amino acids in biological fluids, and determination of amino acids in hydrolyzed protein samples.
These kits are versatile and can be used in applications such as nutritional analysis, pharmaceutical quality control, metabolic profiling, and food composition studies. They provide reliable and reproducible results across various sample types and industries.

2. Are the standards compatible with both LC and LC-MS workflows?
Yes, Waters Amino Acid Analysis Standards & Kits are fully compatible with both LC and LC-MS workflows. They are formulated to work seamlessly with Waters UPLC, HPLC, and LC-MS systems, ensuring accurate quantification and reliable separation. For LC-MS applications, the standards are designed to minimize background interference and enhance sensitivity, making them suitable for targeted or untargeted amino acid profiling.

3. What is included in the Waters Amino Acid Analysis Kits?
Waters Amino Acid Analysis Kits typically include pre-formulated amino acid standards, derivatization reagents, and protocols for sample preparation and analysis. Depending on the kit, it may also contain calibration solutions, buffers, and mobile phase additives optimized for amino acid separations. Some kits are designed for specific applications, such as hydrolyzed protein analysis or metabolic profiling, and include tailored reagents and workflows for these purposes. Detailed instructions and recommended column configurations are also provided for ease of use.

4. Can these kits be used for both free and protein-bound amino acids?
Yes, Waters Amino Acid Analysis Kits can be used for both free and protein-bound amino acids. For free amino acids, samples can be directly analyzed after preparation. For protein-bound amino acids, the kits provide protocols for hydrolysis, which breaks down proteins into individual amino acids prior to analysis. This versatility makes the kits suitable for a variety of applications, from direct metabolite profiling to comprehensive protein analysis in complex samples.