RapiGest SF Surfactant

RapiGest SF Surfactant

Enhance the quality of in-solution enzymatic protein digestions

Enhance the quality of in-solution enzymatic protein digestions

Labs around the globe are trying to simplify digestion protocols and improve throughput of analysis. Waters patented RapiGest SF Surfactant was designed with your lab’s efficiency in mind, significantly decreasing proteolytic digestion time, and enabling same-day digestions with mass spectrometry (MS) compatibility.

RapiGest SF radically enhances the speed and percent recovery of protein enzymatic digestions. This patented anionic surfactant accelerates the in-solution production of peptides generated by proteases, such as Trypsin, Asp-N, Glu-C, Lys-C, and PNGase F. Many hydrophobic proteins are resistant to proteolysis because their cleavage sites are inaccessible to endoproteases. RapiGest SF facilitates the digestion of proteolytically resistant proteins and increases the solubility of hydrophobic proteins and peptides. RapiGest SF is an MS-compatible mild protein denaturant that helps solubilize and unfold proteins, making them more amenable to cleavage without denaturing or inhibiting common proteolytic enzymes like conventional denaturants. This enables use of lesser amounts of expensive endoproteases.

RapiGest SF Protein Digestion Surfactant. RapiGest SF Surfactant
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Specifications

Overview

  • Compatible with liquid chromatography (LC) and MS analysis
  • Enables same-day digestions and analysis
  • Minimal or no post-digestion sample preparation is required
  • Improves efficiency and speed of digestion
  • Simplifies digestion protocols and improves throughput of analysis
  • Does not inhibit enzyme activity, unlike conventional denaturants
  • Allows use of lesser amounts of expensive endoproteases because it is not disruptive of endoprotease activity

Recommended Use: For improving protein sequence coverage and in-solution digestion protocols.

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How RapiGest SF Surfactant works

RapiGest SF Surfactant is a mild denaturant that solubilizes and unfolds the substrate protein, making it more amenable to cleavage. Mild protein denaturation opens the protein structure and exposes the proteolytic sites to enzymatic cleavage. You can accomplish this during a more complex denaturation of globular proteins by heating in a RapiGest SF solution at elevated temperatures.

Compatible with MS

RapiGest SF is an acid labile surfactant that undergoes hydrolysis in acidic conditions. This unique feature allows you to utilize it to remove the surfactant from solutions when desired. Acidic conditions break down RapiGest SF to non-interfering byproducts, therefore peptide ionization is not suppressed.

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RapiGest SF Surfactant

RapiGest SF Surfactant is a powerful, high-efficiency tool designed to enhance in-solution enzymatic protein digestion, particularly in challenging proteomics applications. Whether you are performing mass spectrometry (MS) or liquid chromatography (LC) analysis, RapiGest SF significantly improves digestion efficiency, yielding high-quality results and ensuring reproducibility across various sample types. Its unique formulation allows for better protein solubilization and denaturation, enabling you to tackle even the most complex proteomic workflows with ease and confidence.

RapiGest SF is known for its compatibility with both LC and MS analyses. In typical proteomics workflows, effective protein digestion is a crucial step that can directly influence the quality and reliability of your results. RapiGest SF facilitates faster and more complete digestion by enhancing the accessibility of proteins to proteolytic enzymes, such as trypsin. This leads to higher peptide yields and more consistent data, allowing you to perform more detailed and accurate analyses. Importantly, the surfactant is compatible with downstream MS analysis, ensuring that it does not interfere with sample integrity or affect signal quality.

What makes RapiGest SF especially useful in high-throughput environments is its ability to improve digestion efficiency and streamline workflows. By promoting faster enzymatic activity, the surfactant shortens digestion times, allowing for quicker sample preparation and analysis without compromising quality. This feature is particularly beneficial for labs that need to process large sample sets while maintaining high standards of data reproducibility and consistency. With RapiGest SF, you can be confident that your proteomic experiments will deliver the robust, reliable results you need, even under tight deadlines.

Another key advantage of RapiGest SF is its ability to enhance the solubility of hydrophobic and membrane-bound proteins, which are notoriously difficult to digest. Many traditional surfactants can denature proteins but fail to maintain compatibility with LC and MS workflows. RapiGest SF, on the other hand, is formulated to maintain solubility while ensuring compatibility with these critical analytical techniques. This means you can successfully analyze a wider range of proteins, including those that are typically more resistant to enzymatic digestion.

In addition to improving digestion and solubility, RapiGest SF is fully compatible with various downstream applications, including isotope labeling and quantitative proteomics. This makes it an ideal choice for labs working on complex proteomics projects that require reliable and consistent digestion across diverse sample types. Whether you're analyzing large proteomes or focusing on specific protein subsets, RapiGest SF ensures that you achieve optimal digestion and peptide recovery, enabling more precise quantitation and identification of proteins.

RapiGest SF Surfactant is a critical tool for proteomics labs seeking to improve protein digestion, enhance workflow efficiency, and achieve higher-quality analytical results. By facilitating faster, more efficient enzymatic digestion and ensuring compatibility with LC and MS analyses, RapiGest SF simplifies complex proteomic workflows while maintaining high standards of data integrity and reproducibility.

FAQs about Rapigest SF Surfactant

1. What is Rapigest SF Surfactant?
Rapigest SF Surfactant is a surfactant used to improve the performance of reversed-phase liquid chromatography.

2. What are the benefits of using Rapigest SF Surfactant?
Rapigest SF Surfactant provides improved peak shape, increased resolution, and faster separations.

3. What types of samples can be analyzed with Rapigest SF Surfactant?
Rapigest SF Surfactant can be used to analyze a wide range of samples, including proteins, peptides, and small molecules.

4. How is Rapigest SF Surfactant used?
Rapigest SF Surfactant is typically used as a mobile phase additive in reversed-phase liquid chromatography.

5. Where can I purchase Rapigest SF Surfactant?
Rapigest SF Surfactant is available for purchase from Waters Corporation.