UPLC Intact Mass Analysis Kit with Tubing & Manual
Mass spectrometry has evolved into a powerful tool for analyzing medicinal proteins. Most therapeutic proteins, on the other hand, are kept in a matrix of biological buffers, non-volatile salts, and stabilizers. As a result, one of the most major issues encountered during mass analysis of these proteins is sample processing to eliminate these agents, which frequently produce non-covalent adducts that limit MS responsiveness and complicate the resulting mass spectral data.
Our MassPREP On-Line Desalting Devices offer effective protein desalting, resulting in better LC-MS results, a rapid on-line approach for high-throughput applications, excellent protein recoveries with no detectable carryover, and more than 100 injections from a single cartridge. A MassPREP Micro Desalting Column and an ACQUITY Tubing Kit are included in the UPLC Intact Mass Analysis Application Kit.
The MassPREP on-line desalting column is capable of effectively desalting proteins prior to LC-MS analysis. Because non-volatile salts (e.g., NaCl) can inhibit the ionization of intact proteins, resulting in low detection sensitivity, it is critical to eliminate or considerably reduce their input into the mass analyzer. The reversed-phase phenyl material contained in MassPREP on-line column successfully “traps” proteins, allowing the salts to be flushed to trash prior to protein elution into the mass spectrometer. Cycle times of 4 minutes (for intact antibody) and 10 minutes (for decreased antibody) are possible using an improved LC-MS technique.
The physical properties of the studied proteins, as well as the performance characteristics of the LC-MS system, injector, and injection wash technique utilized, can all influence the results achieved with the UPLC Intact Mass Analysis Application Kit. Following the analytical gradient, further sawtooth gradient cycles can be used to regenerate the column to pre-injection conditions.
You can increase your productivity with the correct lab equipment. You can shop for lab equipment straight from our website and contact us if you have any questions. You may also want to check out the TruView LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk; TruView LCMS Certified vials are manufactured using closely controlled production methods and handling techniques. Mass spectrometers are used to check for cleanliness, and ACQUITY/MS/MS is used to check for low analyte adsorption.
