ProteinWorks eXpress Digest Kit

SKU: 176003689
ProteinWorks eXpress Digest Kit

ProteinWorks eXpress Digest Kit | 176003689


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Product Description

"The ProteinWorks eXpress Digest Start-up Kit simplifies and accelerates the direct digestion of immunopurified plasma & serum samples for LC-MS protein quantification via the surrogate peptide approach. The kit includes pre-measured, lot-traceable reagents, a flexible 96-tube sample collection module, and optimized protocols, offering scientists a standardized, proven route to fast, accurate and precise protein quantification for protein bioanalysis. The ProteinWorks eXpress Digest Start-up Kit contains: (96) Denaturant* Cluster Tubes, Cap Mat & Rack for low protein content samples (~0.2 - 1.0 mg total protein per sample) (12) 8-Strip Caps for Cluster tubes (1) Digestion Buffer (1) Trypsin Dissolving Agent (1) Digestion Inactivation Agent (3) Reduction Agent vials. Each vial is enough for 32 samples (3) Alkylation Agent vials. Each vial is enough for 32 samples (1) Trypsin for low protein content samples (~0.2 - 1.0 mg total protein per sample) *The denaturant is RapiGest™ SF, an enzyme friendly detergent used to denature proteins prior to digestion. For more information on RapiGest SF, see 720000468en."

Specifications

  • Number of Analysis

    96

  • Type

    Sample Preparation Workflow

  • Packages per kit

    2

  • Separation Mode

    RPLC

  • Detection Method

    MS/MS

  • Storage Condition

    Refer to the storage recommendations of each sub-package

  • System Type

    LC-MS

  • Shelf Life

    Refer to the storage recommendations of each sub-package

  • UNSPSC

    41116104

  • Application

    Peptide, Protein

  • Brand

    ProteinWorks

  • Product Type

    Application Kits

  • Units per Package

    1 pk

Product Support

Documents

Documents



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ProteinWorks eXpress Digest Kit

As the therapeutic and research landscape moves from small molecule to large molecule analysis, bioanalytical laboratories search for lab equipment that gives them a competitive edge.

With the use of robust and dependable LC-MS techniques and tools, ProteinWorksTM Sample Preparation Kits enable Discovery and Early Development laboratories to acquire consistent, reproducible, and sensitive protein quantification utilizing the surrogate peptide method. eXpress Digest Kits, a mAb system check and training standard, and an Elution SPE kit for post-digestion cleanup are all available from ProteinWorks. In terms of performance and versatility, the ProteinWorks eXpress Digest Kit is unparalleled.

The Waters ProteinWorksTM eXpress Digest Kits are flexible, broadly applicable sample preparation lab equipment that come with pre-measured, lot-traceable chemicals that are optimized for precise, accurate, and dependable LC-MS protein quantification utilizing the surrogate peptide method.

It might be challenging to obtain sensitivity, reproducibility, standardization, and accuracy while keeping a level of flexibility that supports different LC-MS protein quantification methods. Without the constraints of competing kit-based approaches, the ProteinWorks eXpress Digest Kit is designed for a variety of processes, providing considerable flexibility to generate the best results for your assay needs and the analyte of interest.

The chemicals employed, their concentrations, time, temperature, and the number of stages all have an impact on how sensitive an assay is. It can be challenging to achieve the necessary detection limits. Infliximab can be quickly quantified down to 10 ng/mL using ProteinWorks' universal kit-based sample preparation approach, which includes pre-weighed, lot-traceable reagents and simple, step-by-step instructions.

Workflows for protein quantification are highly variable because of their intricate, multi-segment structure. Assays must abide by authorized accuracy and precision requirements in order to deliver reliable, repeatable results for crucial research decisions. ProteinWorks eXpress Digest Kits offer a streamlined, kit-based technology that enables you to achieve single-digit intra-kit and inter-kit reproducibility across a multitude of monoclonal antibodies.

We recommend that you also explore the Intact mAb Mass Check Standard. The Waters Intact mAb Mass Check Standard is a known molecular weight intact mouse protein purified by Protein-A that is used as an LC/MS standard for qualitative intact mass analysis. It is appropriate for higher molecular weight intact mass measurements.

You will receive the standard in a Waters Max Recovery Vial, which guarantees its high caliber and purity. Performance testing, system setup, and routine MS tuning are all intended uses for this standard. A monoclonal antibody standard with complete characterization is the Intact mAb Mass Check Standard. A monoclonal antibody is a 150 kDa or less molecular weight glycosylated protein.

What Are The Three Techniques For Measuring Proteins In A Sample?

Bicinchoninic acid assay (BCA), various forms of high-performance liquid chromatography (HPLC), and the use of fluorescently or radio-chemically labeled proteins are all examples of protein quantification procedures.