Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
Re-imagine a streamlined, fast, and highly sensitive way to analyze released N-glycans. With just 3 easy steps in as little as 30 minutes, the GlycoWorks RapiFluor-MS N-Glycan Kit reduces complicated, time-consuming sample preparation. RapiFluor-MS's (RFMS) unique chemical attributes provide increased fluorescence quantification and supreme mass spectral response. Now you can use a single label that provides valuable information from characterization to routine monitoring. Glycan analysis just got easier and more sensitive.This core kit includes the GlycoWorks Deglycosylation Module, GlycoWorks Labeling Module, GlycoWorks Clean-up Module, and the GlycoWorks Sample Collection Module.It contains materials to process 24 samples in a 3x8 format.The kit contains:GlycoWorks Deglycosylation Module:(1) 0.035 ml/vial Rapid PNGaseF Enzyme(1) 0.25 ml/vial Glycoworks Rapid Buffer(1) 3 vials of 3 mg/vial RapiGest™ SF Surfactant(1) Intact mAb Mass Check Standard *Shipped in a separate boxGlycoWorks RapiFluor-MS™ Labeling Module:(3) 9 mg/vial RapiFluor-MS Reagent(3) 1 ml/vial Reagent Solvent (Anhydrous DMF)GlycoWorks RFMS Clean-up Module: (4) 0.5 ml/vial Elution Buffer (200mM Ammonium Acetate 95/5 Water/ACN pH 7)(4) 3 ml/vial Sample Diluent (DMF/ACN Mixture)(1) GlycoWorks HILIC µElution PlateGlycoWorks Sample Collection Module:(15) 1 mL tubes for deglycosylation and labeling reaction(15) 600 µL tube inserts for SPE eluate collection(15) Cap strips with slits for direct injection(1) 1 mL round collection tray(1) Waste tray. This kit includes p/n: 186010922 which is a reduced packaging combination of the RFMS Labelling, Clean-up, and Sample Collection modules. p/n: 186010922 cannot be purchased separately and will only be sold as part of a kit.
Specifications
Number of Analysis
24
Type
Sample Preparation Workflow
Packages per kit
5
Separation Mode
HILIC
Detection Method
HRMS, MS, MS/MS, UV
Storage Condition
Refer to the storage recommendations of each sub-package
System Type
LC-MS
Shelf Life
Refer to the storage recommendations of each sub-package
UNSPSC
41116104
Application
Glycan
Brand
GlycoWorks
Product Type
Application Kits
Units per Package
1 pk
Cold ShippingThis part will be shipped cold and shipping and handling fees will be applied to your cart.
As the leader in innovations for biotherapeutic analysis, Waters has reimagined the workflow for released N-glycan analysis. With the novel reagent RapiFluor-MS, it is possible to get the ionization capability necessary to maximize the information generated by MS detection. This new workflow speeds up deglycosylation and streamlines labeling. Biopharmaceutical laboratories no longer have to choose between a fast labeling tool and sensitivity for mass spectrometry or use labor-intensive and complicated processes with room for error. With the GlycoWorks RapiFluor-MS N-Glycan Kit, there are no compromises, just confidence in analytical results.
With just 3 simple steps in as little as 30 minutes, the Glycoworks RapiFluor-MS N-Glycan Kit-96 sample provides unique chemical attributes for increased fluorescence quantification and supreme mass spectral responses.
The core kit includes GlycoWorks Deglycosylation Module, GlycoWorks Labeling Module, GlycoWorks Clean-Up Module, and the GlycoWorks Sample Collection Module. There are materials included to process 24 samples in a 3x8 format.
The lab equipment included in these modules is listed below.
GlycoWorks Deglycosylation Module: (1) 0.035 ml/vial Rapid PNGaseF Enzyme; (1) 0.25 ml/vial Glycoworks Rapid Buffer (1); 3 vials of 3 mg/vial RapiGest™ SF Surfactant (1); Intact mAb Mass Check Standard
GlycoWorks Sample Collection Module: (15) 1 mL tubes for deglycosylation and labeling reaction; (15) 600 µL tube inserts for SPE eluate collection; (15) Cap strips with slits for direct injection; (1) 1 mL round collection tray; (1) Waste tray
What Are The 3 Steps In The GlycoWorks Model?
The procedure commences with a glycoprotein as its starting point. In the initial stage, known as Step 1, completed within a brief timeframe of under 15 minutes, N-glycans are liberated utilizing GlycoWorks Rapid PNGase F, along with Buffer and RapiGest SF Surfactant.
Within a span of 5 minutes, Step 2 involves the labeling of glycans using GlycoWorks RapiFluor-MS Labeling. Moving on to Step 3, which typically takes around 10 minutes, glycans are subjected to purification utilizing either GlycoWorks HILIC µElution Plate or Cartridges.
This simplified procedure streamlines the preparation of labeled, released N-glycan samples, ensuring swift and error-free execution while minimizing sample losses. The straightforward protocol facilitates easy training for analysts.
