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SKU: 186008840 GlycoWorks Rapid Deglycosylation Kit - 4 x 24
The GlycoWorks Rapid Deglycosylation Kits are used to support existing RapiFluor-MS (RFMS) customers who are looking to qualify and validate their deglycosylation efficiency with the protocol. It can also be used for complimentary application areas to compare intact with partial PNGase F deglycosylation for site occupancy information (quick screening) and intact O-linked analysis as shown through application notes in the below references with the Waters ACQUITY UPLC Glycoprotein BEH Amide, 300A column.
The GlycoWorks Rapid Deglycosylation Kit- 4x24 specifically supports the 96 sample protocol and is the same module shipped with the 96 sample kits.
GlycoWorks Deglycosylation Module:
(4) 0.035 ml/vial Rapid PNGaseF Enzyme
(4) 0.25 ml/vial Glycoworks Rapid Buffer
(1) 4 vials of 10 mg/vial RapiGest SF Surfactant
Specifications
Number of Analysis
96
Type
Sample Preparation Workflow
Separation Mode
HILIC
Detection Method
HRMS, MS, MS/MS, UV
Storage Condition
Refer to the storage recommendations of each sub-package
System Type
LC-MS
Shelf Life
Refer to the storage recommendations of each sub-package
UNSPSC
41116104
Application
Glycan
Brand
GlycoWorks
Product Type
Application Kits
Units per Package
1 pk
Cold ShippingThis part will be shipped cold and shipping and handling fees will be applied to your cart.
Use the GlycoWorks Rapid Deglycosylation Kit - 4 x 24 to conduct Glycoprotein analysis that involves identifying complex N- and O-linked structures made up of frequently similar and repeating sugar moieties. The GlycoWorks Rapid Deglycosylation Kit - 4 x 24 is used to support our existing RapiFluor-MS (RFMS) customers who may be looking to qualify and validate their deglycosylation efficiency with the protocol.
Depend on the GlycoWorks Rapid Deglycosylation Kit - 4 x 24 to deliver a convenient, versatile, comprehensive, and effective sample preparation solution for glycan analysis.
The GlycoWorks Rapid Deglycosylation Kit - 4 x 24 can also be used for complementary application areas to compare intact with partial PNGase F deglycosylation for site occupancy information (quick screening) and intact O-linked analysis. It makes use of HILIC (Hydrophilic-interaction liquid chromatography) for separation. HILIC with fluorescence detection is a well-recognized and reliable technique that effectively separates isolated glycans after derivatization with fluorescent labels.
This product listing specifically supports the 96 sample protocol and is the same module shipped with the 96 sample kits. The GlycoWorks Rapid Deglycosylation Kit - 4 x 24 contains 4 vials of 0.035 ml/vial Rapid PNGaseF Enzyme, 4 vials of 0.25 ml/vial Glycoworks Rapid Buffer, and 4 vials of 10 mg/vial RapiGest SF Surfactant. The kit is sufficient to conduct 96 analyses.
The GlycoWorks Rapid Deglycosylation Kit - 4 x 24 enables you to get faster analysis. You can develop convenient and easy-to-use methodologies that enable you to easily move through sample preparation to data collection and interpretation.
Shop for lab equipment directly from our website and also see the complete range of Glycoworks products that have been designed to meet all your analysis needs.
You may also be interested in the GlycoWorks HILIC µElution Plate, which is ideal for removing contaminants like salts and detergents from hydrophilic analytes, such as carbohydrates, prior to mass spectrometry analysis. You can use the µElution Plate by itself for the purpose of extracting carbohydrates, or it can be used as part of our glycan analysis kit to clean up the glycans released from glycoproteins after deglycosylation.
What Are The Applications Of Carbohydrate Detection?
Carbohydrates are important food components that affect taste and nutrition. The determination of the types and concentrations of carbohydrates in foods is essential for energy evaluation, nutritional labeling, quality control, and identifying possible adulteration.
