Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
SKU: 186008841 GlycoWorks Rapid Deglycosylation Kit - 3 x 8
The GlycoWorks Rapid Deglycosylation Kits are used to support existing RapiFluor-MS (RFMS) customers who are looking to qualify and validate their deglycosylation efficiency with the protocol. It can also be used for complimentary application areas to compare intact with partial PNGase F deglycosylation for site occupancy information (quick screening) and intact O-linked analysis as shown through application notes in the below references with the Waters ACQUITY UPLC Glycoprotein BEH Amide, 300A column. The GlycoWorks Rapid Deglycosylation Kit- 3x8 specifically supports the 24 sample protocol that contains enough reagents to complete 3, 8 reaction sample sets.
GlycoWorks Deglycosylation Module:
(1) 0.035 ml/vial Rapid PNGaseF Enzyme
(1) 0.25 ml/vial Glycoworks Rapid Buffer
(3) 3 vials of 3 mg/vial RapiGest™ SF Surfactant
Specifications
Number of Analysis
24
Type
Sample Preparation Workflow
Separation Mode
HILIC
Detection Method
HRMS, MS, MS/MS, UV
Storage Condition
Refer to the storage recommendations of each sub-package
System Type
LC-MS
Shelf Life
Refer to the storage recommendations of each sub-package
UNSPSC
41116104
Application
Glycan
Brand
GlycoWorks
Product Type
Application Kits
Units per Package
1 pk
Cold ShippingThis part will be shipped cold and shipping and handling fees will be applied to your cart.
As part of our mission to remain the leader in innovations for biotherapeutic analysis, Waters has reimagined the workflow for the analysis of released N-Glycans. The novel reagent RapiFluor-MS has made it possible to get the ionization capability necessary to maximize information generated during MS detection. This improved workflow speeds up deglycosylation and streamlines the labeling process, meaning biopharmaceutical scientists no longer have to choose between a fast labeling tool and sensitivity for mass spectrometry or rely on difficult and complicated processes that leave room for error. For those using the RapiFluor-MS solution today, the GlycoWorks Rapid Deglycosylation Kit assists with qualifying and validating deglycosylation efficiency.
The GlycoWorks Rapid Deglycosylation Kit can also be used for complementary application areas to compare intact and partial PNGase F deglycosylation for site occupancy information, and intact O-linked analysis. This is shown in application notes provided by Waters with the ACQUITY UPLC Glycoprotein BEH Amide, 300A column and similar lab equipment that offer wide pore stationary phases and optimized amide bonding. This combination provides unprecedented, MS-compatible HILIC based separations for the analysis of intact glycoproteins, glycoprotein fragments, and glycopeptides.
The kit is also available as a part of full solutions like the Glycoworks RapiFluor-MS N-Glycan Kit- 24 Sample and other similar options. These kits are shipped cold with additional shipping and handling fees applied to your cart.
The GlycoWorks Rapid Deglycosylation Kit 3x8 specifically supports the 24 sample protocol, which contains enough reagents to complete 3, 8 reaction sample sets. The kit includes the following:
(1) 0.035 ml/vial Rapid PNGaseF Enzyme
1) 0.25 ml/vial GlycoWorks Rapid Buffer
(3) 3 vials of 3 mg/vial RapiGest SF Surfactant
What Are Some Common Applications for Deglycosylation?
Deglycosylation and the analysis of released N-glycans are often used in biopharmaceutical laboratories. Reasons to deglycosylate a glycoprotein can include the removal of heterogeneity in glycoproteins for X-ray crystallographic analysis and to remove carbohydrate epitopes from antigens.
Other applications include: enhancement or reduction of blood clearance rates in glycoprotein therapies, investigation of ligand binding, quality control of glycoprotein pharmaceuticals, simplification of analysis of the peptide portion of the glycoprotein, investigation of the role of carbohydrates in enzyme activity and solubility, and simplification of the analysis of the glycan component via glycan sequencing.
