ACQUITY UPLC BEH C18 Column, 130A, 1.7 µm, 2.1 mm X 100 mm, 3/pk
If you are planning to shop for lab equipmentwith unprecedented efficiency, symmetry, and chemical stability, Waters UPLC columns will meet the needs of your lab. One of the industry's premier columns for method development, the 1.7 µm ACQUITY UPLC BEG C18 Column is a universal column choice that works with a diverse range of analytes.
A universal method development tool, this trifunctionally-bonded alkyl column offers industry-leading mobile-phase pH [1-12] and temperature [80 C°] compatibility. Not only can this column provide exceptional stability for extremely high and low pH levels for all types of analytes, but it can improve the retention, selectivity, and sensitivity of ionizable compounds with mobile-phase pH.
Using the innovative UPLC technology, you will benefit from a holistic approach that optimizes both particle technology and instrument design to improve success, accuracy, and productivity in any lab. The UPLC system allows method requirements to be met for a range of separation goals, including speedy analysis, increases in throughput that do not impact resolution, decreased analysis time with improved resolution, or simply superior resolution levels.
With BEH technology particles, this column will give your lab the ability to achieve peak shape and efficiency for basic analyte and a rational array of chromatographic stability, along with chemical stability improvements at mobile phase extremes, especially when there is elevated pH.
The ACQUITY UPLC BEH C18 VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk uses Waters' proprietary end-cap style and is designed specifically for use with the ACQUITY UPLC system. Manufacturing takes place in a cGMP, ISO 9001 certified plant with the use of ultra-pure reagents. Each batch of materials is tested chromatographically with acidic, basic, and neutral analytes with results held to narrow specification ranges. This assures the superior, reproducible performance expected from Waters columns.
FAQs About ACQUITY UPLC BEH C18 Column, 130Å, 1.7 µm, 2.1 mm X 100 mm, 3/pk
1. What are the key benefits of using a 130Å pore size and 1.7 µm particle size?
The 130Å pore size and 1.7 µm particle size of the ACQUITY UPLC BEH C18 Column are optimized for high-resolution and high-efficiency separations. The 130Å pore size is particularly well-suited for analyzing small to mid-sized molecules, providing sufficient pore accessibility for analytes while maintaining excellent peak shape. The 1.7 µm particle size enhances separation efficiency by delivering sharper peaks and greater sensitivity, enabling faster analysis and higher throughput. These features make this column ideal for UPLC applications that require precise and reproducible results.
2. How does the BEH technology improve column durability and lifetime?
The BEH (Ethylene Bridged Hybrid) particle technology used in the ACQUITY UPLC BEH C18 Column ensures exceptional mechanical strength and chemical stability. This advanced material resists high pressures and harsh pH conditions (1-12), allowing for extended column lifetimes even under challenging operating conditions. BEH technology minimizes particle degradation and ensures consistent performance across multiple runs, reducing the frequency of column replacements and improving long-term cost efficiency. Its robustness makes it an excellent choice for both routine analyses and demanding workflows.
3. How do I properly clean and regenerate the column to maintain performance?
To maintain the performance of the ACQUITY UPLC BEH C18 Column, it is essential to follow proper cleaning and regeneration protocols. Flush the column with a series of solvents, starting with a low-strength organic solvent (e.g., 10% acetonitrile in water) to remove any retained polar compounds. Gradually increase the organic solvent concentration to 100% to remove hydrophobic residues.
For persistent contaminants, use a cleaning solution such as 50% methanol or isopropanol with 0.1% formic acid. Always flush the column with its starting mobile phase before resuming analysis to restore equilibrium.
