Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
SKU: 176001132 ACQUITY UPLC HSS T3 Column, 100Å, 1.8 µm, 2.1 mm X 100 mm, 3/pk
ACQUITY UPLC HSS T3 Columns | 2.1 mm X 100 mm | 176001132
The universal, silica-based bonded phase used for the ACQUITY HSS T3 sorbents is compatible with 100% aqueous mobile phase and should be your first choice when developing separations for polar and non-polar compounds. The ACQUITY HSS T3 UPLC columns exhibit ultra-low MS bleed and are fully scalable and transferable to HPLC separations.
ACQUITY UPLC HSS T3 Column, 100Å, 1.8 µm, 2.1 mm X 100 mm, 3/pk
When it comes to retaining and separating polar compounds with reversed-phase HPLC and UPLC technology, ACQUITY UPLC HSS T3 columns have quickly become the new standard amongst chromatographers. Not only do these universal silica-based, reversed-phase C18 columns retain and separate highly polar compounds, but they are able to offer superior performance in a wide pH range. In addition to these benefits, they can also be used in UPLC separations as an additional UPLC chemistry choice.
The benefits of ACQUITY UPLC HSS T3 columns are due to the advanced T3 bonding process developed by Waters. The T3 bonding process makes use of a trifunctional C18 alkyl phase bonded at a ligand density which promotes polar compound retention and aqueous mobile phase compatibility. With the proprietary T3 end-capping process, the columns benefit from a much more effective process than traditional trimethyl silane (TMS) end-capping.
This proprietary combination of bonding and encapping allows for superior polar compound retention and aqueous compatibility, in addition to enhancing column performance, peak shape, stability, and lifetime.
By bonding at a ligand density that is less hydrophobic and more compatible with the weak and highly aqueous mobile phases needed to retain polar compounds, C18 columns that are highly retentive and reversed-phased for polar compound retention. ACQUITY UPLC HSS T3 columns are designed specifically to elongate predictable column lifetimes under low pH conditions without sacrificing polar compound retention and peak shape. The effective and efficient T3 bonding helps to protect the silica substrate from dissolution, contributing to these longer lifetimes.
ACQUITY UPLC HSS T3 columns contain the world’s first and only 100% silica particle that has been designed, tested, and intended for applications up to 15,000 psi/1000 bar. The rugged and unique UPLC silica particle is designed to tolerate the demanding conditions of UPLC separations.
End-capping in chromatography is the replacement of accessible silanol groups in a bonded stationary phase by trimethylsilyl groups. A column that has been end-capped has much lower residual silanol group activity when compared with columns that have not undergone an end-capping process.
End-capping technology helps prevent the tailing of polar compounds’ peaks and shows high durability, even with an alkaline mobile phase, due to the strong film that covers the stationary phase surface. End-capped columns also show decreased retention for hydrogen and bond acceptors, such as ionized bases.
What is UPLC?
UPLC, short for Ultra Performance Liquid Chromatography, represents the progressive advancement in the realm of separation science surpassing HPLC (High Performance Liquid Chromatography). UPLC Columns are meticulously filled with diminutive particles [1.7 - 1.8 µm], empowering laboratories to attain unparalleled levels of chromatographic efficacy. By harnessing UPLC Technology, your laboratory can enhance resolution, sensitivity, and velocity, all without any compromises.
