Chemistry |
Amide |
Separation Mode |
Hydrophilic Interaction (HILIC) |
Particle Substrate |
Hybrid |
pH Range Min |
2 pH |
pH Range Max |
11 pH |
Maximum Pressure |
18000 psi (1240 Bar) |
Endcapped |
No |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
130 Å |
Format |
Column |
Surface Area |
185 |
System |
UHPLC, UPLC |
Particle Technology |
BEH |
USP Classification |
L68 |
Inner Diameter |
2.1 mm |
Length |
100 mm |
Carbon Load |
12 % |
eCord |
Yes |
UNSPSC |
41115709 |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
3 pk |
XBridge BEH Amide Column, 130Å, 2.5 µm, 2.1 mm X 100 mm, 3/pk
The XBridge BEH Amide XP Column has a tri-functionally linked amide phase that is pH stable from 2 to 11, allowing it to separate polar analytes with a wide range of polarity, structural moiety, and pKa. When you're using the XBridge BEH Amide XP Column, your system will retain extremely polar compounds like carbohydrates and sugars. The XBridge BEH Amide XP Column's BEH particle technology provides improved hydrolytic stability and particle strength, eliminating any LC/MS leakage in the system.
The XBridge BEH Amide XP Column is manufactured within packing using ultra-low dispersion hardware and has a particle size of 2.5 m. It is based on the eXtended Performance [XP] column technology, which enables higher separation performance, robustness, and throughput for HPLC investigations while also allowing for a smooth transition to UPLC adoption.
When it comes to HPLC, UHPLC, and UPLC systems, XBridge XP Columns provide a large selection of possibilities. The XBridge BEH Amide XP Column is incredibly customizable, allowing you to quickly design robust procedures by using a variety of mobile phases with a wide pH range. Even when mass loading of basic chemicals is increased, this lab equipment will allow you to generate samples with outstanding chemical stability at high pH and temperatures.
To review additional collections of lab equipment from Waters, you may review our catalog or browse through our website where you can shop for lab equipment to fulfill all your laboratory needs as applicable.
Compatible with the XBridge BEH Amide Column, you may also be interested in XBridge BEH Amide XP VanGuard Cartridge, 130Å, 2.5 µm, 2.1 mm X 5 mm, 3/pk; XBridge BEH Amide VanGuard Cartridges are used to extend analytical column lifetime and performance by removing particulate contamination from the mobile phase stream. This cartridge is optimized to protect all 2.1 mm and 3.0 mm I.D. XBridge BEH Amide analytical columns containing 2.5 µm sorbent particles.
What Is The Significance Of BEH In Chromatography?
The BEH (Ethylene Bridged Hybrid) particle, one of the main enablers underlying UPLC technology, is available in a variety of pore sizes and bonded phases, facilitating both reversed-phase and hydrophilic contact chromatography. The method can be used to assess a wide range of biopharmaceutical substances, including small molecules and large molecules. Hybrid particle technology can be employed in a wider pH range [pH 1-12] since it is chemically stable. As a result, a diverse and dependable separation technology for method development can be developed. BEH particle technology is available in a variety of HPLC particle sizes [2.5, 3.5, 5, and 10 m], allowing for a smooth transfer from HPLC to UPLC platforms.