XBridge Protein BEH SEC, 200 Å Guard Column, containing 3.5um particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 10,000 – 450,000 Daltons. Note: This P/N also contains one vial the BEH200 SEC Protein Std (P/N 186006518) at no additional cost.
Chemistry |
SEC |
Separation Mode |
Gel Filtration (aqueous) |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
3200 psi (221 Bar) |
Endcapped |
No |
Bonding Technology |
Diol (OH) |
Silanol Activity |
Low |
Molecular Weight Range Min |
10000 |
Molecular Weight Range Max |
450000 |
Particle Shape |
Spherical |
Particle Size |
3.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
200 Å |
QC Tested |
Protein |
Format |
Online Column |
Packing Solvent |
20% methanol in water |
Surface Area |
220 |
System |
HPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L33 |
Inner Diameter |
7.8 mm |
Length |
30 mm |
Carbon Load |
12 % |
eCord |
No |
UNSPSC |
41115711 |
Application |
Protein |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge Protein BEH SEC Guard Column, 200Å, 3.5 µm, 7.8 mm X 30 mm, 1/pk and BEH200 SEC Protein Standard Mix
The Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating used in our successful range of HPLC-based SEC columns are employed in the XBridge Protein BEH SEC, 200 Guard Column, featuring 3.5um particles. They are best for separating proteins with a Daltons range of 10,000 to 450,000. Unlike silica-based SEC Columns, the XBridge Protein BEH SEC Column has fewer non-desired protein and column interactions, enabling the column to have a longer column life.
This offering also includes a free vial of the BEH200 SEC Protein Standard Mix, which is a comprehensive protein combination with five components: thyroglobulin, IgG, BSA, Myoglobin, and Uracil. While running your tests, this standard is recommended for benchmarking column and system performance. This can be used to test ACQUITY UPLC BEH200 SEC, 1.7 m Columns, as well as the XBridge Protein BEH SEC Column.
The XBridge Protein BEH SEC Column's diol-bonded covering provides greater flow and pressure tolerance for increased sample throughput on HPLC systems. The combination of BEH base particle technology and diol bonding methods allows for better column stability, performance, and lifetime.
The XBridge Protein BEH SEC Column allows for a smooth transition to UltraPerformance LC Technology, and this equipment is intended to give maximum productivity. The XBridge Protein BEH SEC Column, like all Waters equipment, is dependable and reproducible because it is manufactured in cGMP, ISO9001, and ISO13485 certified facilities. Every piece of equipment goes through thorough testing at every stage and phase of production to ensure the best possible quality. The column is an important piece of laboratory equipment because it has unrivaled column longevity and the ability to function with a wide variety of mobile phase pH and temperatures.
Shop for lab equipment, and find our complete range of XBridge columns across a wide range of applications through the catalog on our website. You may also be interested in XBridge Protein BEH SEC Column, 200Å, 3.5 µm, 7.8 mm X 150 mm, 1/pk and BEH200 SEC Protein Standard Mix; XBridge Protein BEH SEC, 200 Å Columns, containing 3.5um particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns.
What pH Range Should The XBridge Protein BEH 200 SEC Column Be Used In?
The optimal pH range for the XBridge Protein BEH 200 SEC Column is 2-8. Please bear in mind, employing higher pH may affect the performance and lifetime of your column.