SKU: 176003598
XBridge Protein BEH SEC Column, 450 Å, 3.5 µm, 7.8 mm X 150 mm, 1/pk and BEH450 SEC Protein Standard Mix

XBridge Protein BEH SEC Column | 176003598


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Product Description

XBridge Protein BEH SEC, 450 Å Columns, containing 2.5um particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 100,000 – 1,500,000 Daltons. Note: This item in shipped with one vial the BEC450 SEC Protein Std  at no additional cost (PN 186006842).

Specifications

  • Chemistry

    SEC

  • Separation Mode

    Gel Filtration (aqueous)

  • Particle Substrate

    Hybrid

  • pH Range Min

    2.5 pH

  • pH Range Max

    8 pH

  • Temperature Limits

    60 C

  • Maximum Pressure

    3000 psi (207 Bar)

  • Endcapped

    No

  • Bonding Technology

    Diol (OH)

  • Silanol Activity

    Low

  • Molecular Weight Range Min

    100000

  • Molecular Weight Range Max

    1500000

  • Particle Shape

    Spherical

  • Particle Size

    3.5 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    450 Å

  • QC Tested

    Protein

  • Format

    Online Column

  • Packing Solvent

    20% methanol in water

  • Surface Area

    80

  • System

    UPLC

  • Particle Technology

    BEH

  • Technique

    LC

  • USP Classification

    L33

  • Inner Diameter

    7.8 mm

  • Length

    150 mm

  • Carbon Load

    9 %

  • eCord

    No

  • UNSPSC

    41115711

  • Application

    Protein

  • Brand

    XBridge

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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XBridge Protein BEH SEC Column, 450 Å, 3.5 µm, 7.8 mm X 150 mm, 1/pk and BEH450 SEC Protein Standard Mix

The Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating used in our successful series of HPLC-based SEC columns are employed in the XBridge Protein BEH SEC, 450 Å Columns, which contain 2.5µm particles. They are best suited for separating proteins with a size range of 100,000 to 1,500,000 Daltons, and The XBridge Protein BEH SEC Column and BEH450 SEC Protein Standard Mix were designed to meet even the most stringent chromatographic requirements.

BEH base particle technology combined with diol bonding methods improves column stability, performance, and longevity. The XBridge Protein BEH SEC Column comes with a vial of the BEH450 SEC Protein Standard Mix. The BEH450 SEC Protein Standard Mix should be utilized as a benchmark for column and system performance. The diol-bonded covering of the XBridge Protein BEH SEC Column allows for improved flow and pressure tolerance for increased sample throughput on HPLC systems.

The XBridge Protein BEH SEC Column facilitates the shift to UltraPerformance LC Technology and is designed to maximize productivity. Because it is manufactured in cGMP, ISO9001, and ISO13485 certified facilities, the XBridge Protein BEH SEC Column, like all Waters equipment, is dependable and reproducible. Every piece of equipment is thoroughly tested at every level and phase of manufacture to ensure the highest quality possible. Because of its unequaled column longevity and ability to perform with a wide range of mobile phase pH and temperatures, the column is an important piece of laboratory equipment.

Visit our website to explore our complete line of XBridge columns and to shop for lab equipment that matches your individual needs. Please contact a member of our global team if you require assistance with any equipment or have any questions.

You might also want to look into TruView LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk; TruView LCMS Certified vials are manufactured using highly controlled manufacturing and handling techniques. Mass spectrometers are used to check for cleanliness, and ACQUITY/MS/MS is used to check for low analyte adsorption.

How Much Of A pH Range Can The XBridge Protein BEH SEC Column And BEH450 SEC Protein Standard Mix Operate Under?

The XBridge Protein BEH SEC Column and BEH450 SEC Protein Standard Mix work well in the 2.5 - 8 pH range, at 60 C, and at a maximum pressure of 3000 psi (or 207 Bar). However, we do not recommend exceeding these pH, pressure, or temperature ranges because it may have an influence on the performance and durability of your column.