SKU: 176003904
ACQUITY UPLC Protein BEH SEC Column, 200 Å, 1.7 µm, 4.6 mm x 150 mm, 1/pk and BEH200 SEC Protein Standard Mix

ACQUITY UPLC Protein BEH SEC Column | 176003904

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Product Description

Based upon the highly successful BEH particle technology, the ACQUITY UPLC Protein BEH SEC Column, 200 Å, 1.7 µm, 4.6 mm x 150 mm represents the first commer­cially available sub-2-µm SEC chemistry. Fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 10000 to 450,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods.   Note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.

Specifications

  • Chemistry

    SEC

  • Separation Mode

    SEC

  • Particle Substrate

    Hybrid

  • pH Range Min

    2.5 pH

  • pH Range Max

    8 pH

  • Temperature Limits

    60 C

  • Maximum Pressure

    7000 psi (483 Bar)

  • Endcapped

    No

  • Bonding Technology

    Diol (OH)

  • Silanol Activity

    Low

  • Molecular Weight Range Min

    10000

  • Molecular Weight Range Max

    450000

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    200 Å

  • QC Tested

    Protein

  • Format

    Column

  • Packing Solvent

    20% methanol in water

  • Surface Area

    220

  • System

    UHPLC, UPLC

  • Particle Technology

    BEH

  • Technique

    SEC

  • USP Classification

    L33

  • Inner Diameter

    4.6 mm

  • Length

    150 mm

  • Carbon Load

    12 %

  • eCord

    Yes

  • UNSPSC

    41115709

  • Application

    Protein

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents

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FAQs about Acquity UPLC Protein BEH SEC Column

What is the recommended flow rate for the Acquity UPLC Protein BEH SEC Column?
The recommended flow rate for the Acquity UPLC Protein BEH SEC Column is 0.3 mL/min.

What is the particle size of the Acquity UPLC Protein BEH SEC Column?
The Acquity UPLC Protein BEH SEC Column has a particle size of 1.7 μm.

What is the column dimensions of the Acquity UPLC Protein BEH SEC Column?
The column dimensions of the Acquity UPLC Protein BEH SEC Column are 4.6 mm x 150 mm.

What is the recommended temperature range for operating the Acquity UPLC Protein BEH SEC Column?
The recommended temperature range for operating the Acquity UPLC Protein BEH SEC Column is 30-60°C.

What is the maximum pressure limit for the Acquity UPLC Protein BEH SEC Column?
The Acquity UPLC Protein BEH SEC Column has a maximum pressure limit of 15,000 psi.

How Do I Maximize The Lifetime Of My ACQUITY UPLC Protein BEH SEC Column?

With proper care and handling, these columns can deliver a stable performance for over one thousand sample injections. However, the performance and lifetime will deteriorate if the samples and mobile phases introduced into the column contain particulates. In order to maximize column lifetime, high-quality filter water should be used, with mobile phase buffers passed through a filter of less than 0.2 µm. It is also recommended that mobile phase buffers be filtered using a sterile disposable filter. Sintered glass filter supports should be avoided if filtering mobile phases due to the potential of introducing silicates which could alter performance.