Based upon the highly successful BEH particle technology, the ACQUITY UPLC Protein BEH SEC Column, 200 Å, 1.7 µm, 4.6 mm x 300 mm represents the first commercially available sub-2-µm SEC chemistry. Fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 10000 to 450,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods. Note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.
Chemistry |
SEC |
Separation Mode |
SEC |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
10000 psi (690 Bar) |
Endcapped |
No |
Bonding Technology |
Diol (OH) |
Silanol Activity |
Low |
Molecular Weight Range Min |
10000 |
Molecular Weight Range Max |
450000 |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Parker-style |
Pore Size |
200 Å |
QC Tested |
Protein |
Format |
Column |
Packing Solvent |
20% methanol in water |
Surface Area |
200 |
System |
UHPLC, UPLC |
Particle Technology |
BEH |
Technique |
SEC |
USP Classification |
L33 |
Inner Diameter |
4.6 mm |
Length |
300 mm |
Carbon Load |
12 % |
eCord |
Yes |
UNSPSC |
41115709 |
Application |
Protein |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC Protein BEH SEC Column, 200Å, 1.7 µm, 4.6 mm x 300 mm, 1/pk and BEH200 SEC Protein Standard Mix
Use the combination pack of ACQUITY UPLC Protein BEH SEC Column and BEH200 SEC Protein Standard Mix to meet your protein analysis needs. The ACQUITY UPLC Protein BEH SEC Column enables you to analyze a wide range of proteins while the BEH200 SEC Protein Standard Mix is designed to help you benchmark your system’s performance prior to carrying out important investigations.
The patent-pending ACQUITY UPLC Protein BEH SEC Column is the first commercially available in the sub 2µm SEC chemistry, setting a new industry standard for protein analysis. It is ideally suited to be the reliable lab equipment for the analysis of proteins and their aggregates with molecular weights ranging from 10,000 to 450,000 Daltons. The dependable analytical column will consistently and routinely deliver fast, accurate aggregate determinations up to 10 times quicker than traditional HPLC-based SEC methods.
With the ACQUITY UPLC Protein BEH SEC Column, you will observe outstanding improvements in resolution, throughput, and sensitivity for assays. It will make it possible to follow the presence of protein aggregates through size-exclusion chromatography (SEC).
The BEH200 SEC Protein Standard Mix consists of 5 component protein mixtures including Thyroglobulin, IgG, BSA, Myoglobin, and Uracil that are used to benchmark the ACQUITY UPLC Protein BEH SEC Column before any analysis is conducted. Waters recommended the use of this standard on a regular basis for benchmarking column and system performance, similar to the QCRM product line.
Shop for lab equipment, and browse through our complete product list. You may also want to read up on the technology used in the designing and manufacturing of our products.
You may also want to explore the ACQUITY UPLC Protein BEH SEC Guard Column, 200Å, 1.7 µm, 4.6 mm X 30 mm, 10K - 500K, 1/pk. The Guard Column is ideally suited to the analytical column as it is manufactured using the identical packing material and has been designed specially to protect the column from harm. This is achieved by removing all chemical and particulate contamination from entering the mobile phase of the column. Thus, it ensures that your analysis always has accurate and reliable results, and your analytical column lasts a long time.
Why Is It Important To Benchmark Your System Before Analysis?
Conducting a benchmarking test prior to conducting your analysis will help you determine the optimum levels your system can perform at, and thus, measure whether your equipment is giving accurate results.