Based upon the highly successful BEH particle technology, the ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm x 150 mm represents the first commercially available sub-2-µm SEC chemistry. Fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 1000 to 80,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods. Note: This column includes the BEH125 SEC Protein Standard (PN: 186006519) at no additional cost. Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.
Chemistry |
SEC |
Separation Mode |
SEC |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
10000 psi (690 Bar) |
Endcapped |
No |
Bonding Technology |
Diol (OH) |
Silanol Activity |
Low |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
80000 |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Parker-style |
Pore Size |
125 Å |
QC Tested |
Protein |
Format |
Column |
Packing Solvent |
20% methanol in water |
Surface Area |
220 |
System |
UHPLC, UPLC |
Particle Technology |
BEH |
Technique |
SEC |
USP Classification |
L33 |
Inner Diameter |
4.6 mm |
Length |
150 mm |
Carbon Load |
12 % |
UNSPSC |
41115709 |
Application |
Protein |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm x 150 mm, 1/pk and BEH125 SEC Protein Standard Mix
Add the ACQUITY UPLC Protein BEH SEC Column to your lab equipment collection as it is based upon the highly successful BEH particle technology and is the first commercially available sub-2-µm SEC chemistry. The lab equipment has been designed such that it is fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 1000 to 80,000 Daltons. The total system solution will routinely deliver fast, accurate aggregate determinations that are up to 10 times quicker than the traditional HPLC-based SEC methods. For optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume. This particular listing is a kit that includes the BEH125 SEC Protein Standard Mix (PN: 186006519) at no additional cost.
By utilizing the ACQUITY UPLC Protein BEH SEC Column, you can take advantage of some key features such as exceptional improvements in resolution, throughput, and sensitivity for assays. The lab equipment enables you to monitor the presence of protein aggregates through size-exclusion chromatography (SEC), which in turn increases the efficacy as the presence of these proteins can compromise the safety and efficacy of biologic-based therapeutics.
This combination kit of the ACQUITY UPLC Protein BEH SEC Column column contains the BEH450 SEC Protein Standard Mix, which is a 5 component protein mixture (Thyroglobulin, IgG, BSA, Myoglobin, Uracil). The Standard Mix has the crucial function of enabling the benchmarking of the ACQUITY UPLC BEH450 SEC, 2.5 µm Columns. This makes it possible for you to conduct regular column benchmarking and system performance checks. It is recommended that these checks be made part of the routine checkups to ensure all equipment is performing adequately.
We recommend that you protect your analytical column by using the ACQUITY UPLC Protein BEH SEC Guard Column, 125 Å, 1.7 µm, 4.6 mm X 30 mm, 1/pk. The guard column prevents any harmful contaminants from entering the mobile stream, thus improving the efficiency of the column as well.
What Is The Maximum Pressure That The ACQUITY UPLC Protein BEH SEC Column Can Withstand?
The ACQUITY UPLC Protein BEH SEC Column listed here is designed to be able to tolerate up to a maximum pressure of 10,000 psi (690 Bar).