Based upon the highly successful BEH particle technology, the ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm x 300 mm represents the first commercially available sub-2-µm SEC chemistry. Fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 1000 to 80,000 Daltons, the total system solution will routinely deliver fast, accurate aggregate determinations up to 10 times quicker that traditional HPLC-based SEC methods. Note: This column includes the BEH125 SEC Protein Standard (PN: 186006519) at no additional cost. Also note that for optimal SEC separation performance, this column is designed for use in UPLC SEC applications with an appropriate low LC system volume.
Chemistry |
SEC |
Separation Mode |
SEC |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
7000 psi (483 Bar) |
Endcapped |
No |
Bonding Technology |
Diol (OH) |
Silanol Activity |
Low |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
80000 |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Parker-style |
Pore Size |
125 Å |
QC Tested |
Protein |
Format |
Column |
Packing Solvent |
20% methanol in water |
Surface Area |
220 |
System |
UHPLC, UPLC |
Particle Technology |
BEH |
Technique |
SEC |
USP Classification |
L33 |
Inner Diameter |
4.6 mm |
Length |
300 mm |
Carbon Load |
12 % |
UNSPSC |
41115709 |
Application |
Protein |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC Protein BEH SEC Column, 125Å, 1.7 µm, 4.6 mm x 300 mm, 1/pk and BEH125 SEC Protein Standard Mix
Get the ultimate analysis pack with the ACQUITY UPLC Protein BEH SEC Column and BEH125 SEC Protein Standard Mix. The single pack contains both the analytical column and the standard mix that has been designed to benchmark the performance of the column.
With the patent-pending Waters ACQUITY UPLC Protein BEH SEC Column, you can routinely deliver fast, accurate aggregate determinations up to 10 times quicker than traditional HPLC-based SEC methods. The lab equipment is based upon the highly successful BEH particle technology and is the first commercially available sub 2 µm SEC chemistry. In addition to this, the ACQUITY UPLC Protein BEH SEC Column has been fully optimized for the analysis of proteins and their aggregates with molecular weights ranging from 1000 to 80,000 Daltons. The analytical column has been optimized for the analysis of proteins and their aggregates to offer robust, fast, and accurate determinations.
To ensure that your ACQUITY UPLC Protein BEH SEC Column is performing at its best, use the BEH125 SEC Protein Standard Mix to benchmark its performance. The protein standard contains 4 component protein mixtures, Thyroglobulin, Ovalbumin, Ribonuclease A, and Uracil. Waters recommends the use of this standard periodically for benchmarking column and system performance, similar to the QCRM product line.
The ACQUITY UPLC Protein BEH SEC Column also delivers exceptional improvements in resolution, throughput, and sensitivity for assays. It enables you to monitor the presence of protein aggregates through size-exclusion chromatography (SEC). This results in increased efficacy as the presence of these proteins can compromise the safety and efficacy of biologic-based therapeutics.
The ACQUITY UPLC Protein BEH SEC Column uses packing materials that were designed specifically for use with the ACQUITY UPLC System. The lab equipment is manufactured in a cGMP, ISO 9001 certified plant using ultra-pure reagents, like all Waters manufactured products. The packing material goes through extensive chromatographic tests with acidic, basic, and neutral analytes, and the results are held to narrow specification ranges to assure excellent, reproducible performance. Every ACQUITY UPLC Protein BEH SEC Column is also tested and a Performance Chromatogram and Certificate of Batch Analysis are provided on the eCord Intelligent Chip so you can rely on the quality to ensure you get the most reliable equipment.
To shop for lab equipment, browse through our website or catalog. You may want to explore products like theXBridge Protein BEH SEC Column or other related pieces of lab equipment.
How Many Types Of Proteins Are There?
Proteins are macromolecules and can be classified into four different levels of structure, namely, primary, secondary, tertiary, and quaternary.