XBridge Protein BEH SEC, 200 Å Columns, containing 2.5um particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 10,000 – 450,000 Daltons. Note: This P/N also contains one vial the BEH200 SEC Protein Std (P/N 186006518) at no additional cost.
Chemistry |
SEC |
Separation Mode |
Gel Filtration (aqueous) |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
4500 psi (310 Bar) |
Endcapped |
No |
Bonding Technology |
Diol (OH) |
Silanol Activity |
Low |
Molecular Weight Range Min |
10000 |
Molecular Weight Range Max |
450000 |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
200 Å |
QC Tested |
Protein |
Format |
Online Column |
Packing Solvent |
20% methanol in water |
Surface Area |
220 |
System |
UPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L33 |
Inner Diameter |
4.6 mm |
Length |
150 mm |
Carbon Load |
12 % |
eCord |
No |
UNSPSC |
41115711 |
Application |
Protein |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge Protein BEH SEC Column, 200 Å, 2.5 µm, 4.6 mm X 150 mm, 1/pk
Because it has fewer undesired protein-column interactions, the Waters XBridge Protein BEH SEC Column has a longer column life than silica-based SEC columns. In addition to this, the lab equipment improves throughput and allows HPLC-based SEC resolution of proteins ranging from 10,000 to 450,000 Daltons. The diol-bonded XBridge Protein BEH SEC Columns' flow and pressure tolerance ewenables for increased sample throughput on HPLC equipment. They use BEH base particle technology with innovative diol bonding processes to give superior stability, performance, and longevity.
Invest in the XBridge Protein BEH SEC, 200 Column, which features 2.5µm particles from Waters' Ethylene Bridged Hybrid (BEH)-based particle technology with a diol-bonded surface coating similar to that found in HPLC-based SEC columns.
You can simply develop and transfer SEC methods based on lab equipment using the XBridge Protein BEH SEC Column. You can also build techniques depending on the resolution of required protein components and sample throughput requirements using the lab equipment. The XBridge Protein BEH SEC Columns were created to supplement Waters' existing line of smaller particle size UPLC-based SEC columns for size-exclusion chromatography of proteins.
Because it is manufactured in cGMP, ISO9001, and ISO13485 certified Waters facilities, the XBridge Protein BEH SEC Column, like other lab equipment created and delivered by Waters, ensures consistent and reliable performance. Through maintaining unequaled column consistency and your confidence in validation processes, extensive testing is undertaken at each stage, from packing materials to post-production.
The Waters XBridge Protein BEH SEC Column has fewer undesired protein-column interactions than silica-based SEC columns. As a result, the column will last a long time and not need to be replaced periodically. On HPLC systems, the diol-bonded XBridge Protein BEH SEC Column enables for increased sample throughput while maintaining flow and pressure tolerance. By merging BEH base particle technology with innovative diol bonding processes, column stability, performance, and longevity can all be improved.
You may also want to explore and invest in the XBridge Protein BEH SEC Guard Column, 200 Å, 2.5 µm, 4.6 mm X 30 mm, 1/pk and BEH200 SEC Protein Standard Mix.
What pH Range Does The XBridge Protein BEH SEC Column?
This particular XBridge Protein BEH SEC Column works well across the 2.5 - 8 pH range.