XBridge Protein BEH SEC, 200 Å Columns, containing 2.5um particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns. They are best suited to separate proteins ranging from 10,000 – 450,000 Daltons. Note: This P/N also contains one vial the BEH200 SEC Protein Std (P/N 186006518) at no additional cost.
Chemistry |
SEC |
Separation Mode |
Gel Filtration (aqueous) |
Particle Substrate |
Hybrid |
pH Range Min |
2.5 pH |
pH Range Max |
8 pH |
Temperature Limits |
60 C |
Maximum Pressure |
6,500 psi (448 Bar) |
Endcapped |
No |
Bonding Technology |
Diol (OH) |
Silanol Activity |
Low |
Molecular Weight Range Min |
10000 |
Molecular Weight Range Max |
450000 |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
200 Å |
QC Tested |
Protein |
Format |
Online Column |
Packing Solvent |
20% methanol in water |
Surface Area |
220 |
System |
UPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L33 |
Inner Diameter |
4.6 mm |
Length |
300 mm |
Carbon Load |
12 % |
eCord |
No |
UNSPSC |
41115711 |
Application |
Protein |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge Protein BEH SEC Column, 200Å, 2.5 µm, 4.6 mm X 300 mm, 1/pk and BEH200 SEC Protein Standard Mix
To satisfy even the most exacting chromatographic requirements, the XBridge Protein BEH SEC Column and BEH200 SEC Protein Standard Mix were developed. With the XBridge Protein BEH SEC Column and BEH200 SEC Protein Standard Mix, you may obtain exceptional, mobile-phase pH stability, extending column lifespan. These multi-purpose columns can be used to build a quality control approach or to create a cutting-edge LC-MS assay. The XBridge Protein BEH SEC Column and BEH200 SEC Protein Standard Mix have been created to boost productivity in your lab.
It is advised for separating proteins in the 10,000–450,000 Daltons range to use the XBridge Protein BEH SEC Column, which is based on Ethylene Bridged Hybrid (BEH)-based particle technology with dial-bonded surface coating. In order to guarantee that you receive the best goods for your laboratory and that you can rely on the equipment to give precision-focused results, Waters equipment is put through a variety of inspections and individual tests in our specialized facilities. To guarantee that the highest quality standards are met, these facilities follow stringent industrial processes, and the equipment is made in accordance with ISO9001 and cGMP regulations.
The XBridge Protein BEH SEC Column, which guarantees the robustness of assays and offers unparalleled peak particle shape and capacity with remarkable efficiency, will deliver outstanding column reliability in your system. The XBridge Protein BEH SEC Column comes with a vial of the BEH200 SEC Protein Standard Mix. The BEH200 SEC Protein Standard Mix should be used as a gauge for measuring the effectiveness of columns and systems.
Visit our website to view the entire selection of XBridge columns and to find the lab supplies that best suit your needs and shop for lab equipment. Please get in touch with a member of our international team if you need help with any equipment or if you have any questions.
You might want to look into TruView LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk; Vials that are TruView LCMS Certified are made using strictly regulated manufacturing and handling techniques. Mass spectrometers are used to check for cleanliness, and ACQUITY/MS/MS is used to check for low analyte adsorption.
What pH Range Can The BEH200 SEC Protein Standard Mix And The XBridge Protein BEH SEC Column Operate Under?
The ideal operating conditions for the XBridge Protein BEH SEC Column and BEH200 SEC Protein Standard Mix are a pH range of 2.5 to 8, a maximum temperature of 60 C, and a maximum pressure of 6,500 psi (448 Bar). The performance and lifetime of your column may be impacted by utilizing higher pH, pressure, or temperatures than these ranges; hence we do not advise doing so.