Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
SKU: 176004595 BioResolve SEC mAb Column, 200Å, 2.5 µm, 7.8 x 300 mm, 1/pk with mAb Size Variant Standard
The BioResolve SEC mAb 7.8 x 150 mm Column contains 2.5 µm BEH particle technology and is application specifically tested with the mAb Size Variant Standard to ensure accurate and highly reproducible quantitation of monoclonal antibody (mAb) monomers from frequently associated high molecular weight aggregates (≥300,000 Da) and lower molecular weight fragments (e.g., ≤100,000 Da). This column is also shipped with Water mAb Size Variant Standard at no extra charge (PN 176004593)
Specifications
Chemistry
SEC
Separation Mode
SEC
Particle Substrate
Hybrid
pH Range Min
2.5 pH
pH Range Max
8 pH
Temperature Limits
60 C
Maximum Pressure
6,500 psi (448 Bar)
Endcapped
No
Bonding Technology
Diol (OH)
Silanol Activity
Low
Molecular Weight Range Min
10000
Molecular Weight Range Max
450000
Particle Shape
Spherical
Particle Size
2.5 µm
Endfitting Type
Parker-style
Pore Size
200 Å
QC Tested
Protein
Format
Online Column
Packing Solvent
10% acetonitrile/90% 25 mM sodium phosphate pH 7.0 + 100 mM KCl
BioResolve SEC mAb Column, 200Å, 2.5 µm, 7.8 x 300 mm, 1/pk with mAb Size Variant Standard
In order to ensure accurate and highly reproducible quantitation of monoclonal antibody (mAb) monomers from frequently associated high molecular weight aggregates (300,000 Da) and lower molecular weight fragments (e.g., 100,000 Da), the BioResolve SEC mAb 7.8 x 150 mm Column contains 2.5 m BEH particle technology. Additionally, Water mAb Size Variant Standard is delivered with this column at no additional cost (PN 176004593).
The BioResolve SEC mAb Column is developed in specialized water facilities in accordance with cGMP and ISO 9001 certified production conditions. These facilities use ultra-pure reagents, adhere to the strictest manufacturing methods, and uphold the highest industry standards. A variety of QC tests are used to evaluate each batch of the BioResolve SEC mAb Column with mAb Size Variant Standards. The Waters mAb Size Variant Standard is used to assess each piece of lab equipment for effectiveness and mAb performance in order to give you batch-over-batch and column-to-column consistency for the tough and complex mAb application.
The Water mAb Size Variant Standard (PN 176004592) is also available with this specific lab apparatus at no additional cost. Please look through our catalog or website to learn more about the various lab equipment that Waters manufactures and produces. In addition to connecting with a member of our global support staff who can help you with any inquiries or worries you might have, you will be able to shop for lab equipment in accordance with your laboratory needs.
You might also be interested in the mAb Size Variant Standard; Waters mAb Size Variant Standard (p/n: 186009429), which includes the ‘NIST’ humanized monoclonal antibody (Reference Material 8671) and non-reduced IdeS digested NIST mAb fragments F(ab')2 (100,000 Da) and (Fc/2)2 (50,000 Da), may also be of interest to you. Waters mAb size variant standard can be used to assess column and LC System capability to separate mAb aggregates, monomer, and fragments/clips using SEC by aliquoting small, standard amounts of IdeS fragments.
What Are The BioResolve SEC mAb Column's Operational Limitations With Regard To The mAb Size Variant Standard?
Operating conditions for the BioResolve SEC mAb Column with mAb Size Variant Standard include a pH range of 2.5 to 8, a temperature range of 60 C, and a maximum pressure of 6500 psi (or 448 Bar). The BioResolve SEC mAb Column with BioResolve SEC mAb Guard has a molecular weight range of 10,000 to up to 450,000.
