XBridge Premier Protein SEC Column, 250Å, 2.5 µm, 4.6 x 300 mm with mAb Size Variant Standard, 1/pk

The XBridge Premier Protein SEC Column, 250Å, 2.5 µm, 4.6 x 300 mm with mAb Size Variant Standard, offers cutting-edge technology for reliable and efficient protein size variant separations. Designed specifically for high-performance liquid chromatography (HPLC) applications, this column enables scientists to achieve precise and reproducible results when analyzing proteins, monoclonal antibodies, and other biomolecules. With its advanced features and included Waters mAb Size Variant Standard, it streamlines workflows and enhances confidence in analytical outcomes.

This SEC (size exclusion chromatography) column features a 250Å pore size and 2.5 µm particle size, making it highly effective in separating proteins ranging from approximately 10,000 to 650,000 Daltons. Its column dimensions of 4.6 x 300 mm provide optimal resolution, ensuring detailed insights into complex biomolecules. The XBridge Premier Protein SEC Column leverages Waters" innovative MaxPeak Premier High Performance Surfaces and BEH-PEO particle technologies. These advanced materials minimize undesired secondary interactions, such as ionic or hydrophobic interactions between the proteins and the column surface, allowing for cleaner, more accurate separations.

One of the key advantages of this column is its ability to reduce method development time and complexity. By virtually eliminating secondary interactions, chromatographers can use a "generic" or "platform-type" method for a broad range of samples. This flexibility eliminates the need for extensive optimization for each specific protein, saving valuable time and resources in the laboratory. Whether analyzing therapeutic monoclonal antibodies or characterizing protein aggregates, the XBridge Premier Protein SEC Column delivers reproducible results with exceptional performance.

The column is provided with a vial of Waters mAb Size Variant Standard (p/n: 186009429) at no additional cost. This standard serves as a valuable tool for calibration, ensuring accurate and consistent results during protein size variant analysis. The inclusion of this standard further supports method validation and boosts confidence in data quality, making the column an ideal choice for regulated environments such as biopharmaceutical development and quality control.

The XBridge Premier Protein SEC Column is compatible with a wide range of mobile phases, further enhancing its versatility. Its design is robust and reliable, making it suitable for high-throughput workflows and challenging sample matrices. The column"s ability to maintain high resolution and sensitivity ensures that even small differences in molecular weight or size variants are accurately detected, providing crucial insights for researchers and analysts.

Applications for the XBridge Premier Protein SEC Column are extensive and include the characterization of monoclonal antibodies (mAbs), the study of protein aggregates and fragments, and general protein size profiling. In biopharmaceutical development, where precise characterization of therapeutic proteins is critical, this column enables researchers to confidently assess size heterogeneity, ensuring product consistency and regulatory compliance.

Waters" MaxPeak Premier technology and BEH-PEO particle design not only deliver superior separation performance but also extend the column"s lifespan by reducing surface fouling and degradation. This ensures long-term cost efficiency and reliable performance, even in demanding analytical conditions.

The XBridge Premier Protein SEC Column, 250Å, 2.5 µm, 4.6 x 300 mm with mAb Size Variant Standard, is a powerful tool for scientists seeking reliable and efficient protein size variant separations. With its advanced materials, minimized secondary interactions, and included calibration standards, it simplifies workflows, reduces method development time, and delivers consistent, high-quality results.

FAQs About XBridge Premier Protein SEC Column, 250Å, 2.5 µm, 4.6 x 300 mm with mAb Size Variant Standard

1. What are the benefits of the BEH-PEO particle technology used in the column?
The BEH-PEO particle technology in the XBridge Premier Protein SEC Column is designed to significantly enhance separation performance by reducing non-specific interactions between proteins and the column surface. This technology provides a highly stable, hydrophilic environment that minimizes ionic and hydrophobic interactions, ensuring clean, accurate separations even with challenging protein samples.

2. What mobile phases are compatible with the XBridge Premier Protein SEC Column?
The XBridge Premier Protein SEC Column is compatible with a wide range of mobile phases, offering flexibility to suit various protein and biomolecule analyses. Typical mobile phases include aqueous buffers with neutral pH, as well as additives like sodium phosphate, sodium chloride, or mild detergents to stabilize proteins and prevent aggregation.