DEAE functionalized non-porous resin for high resolution oligonucleotide separations.

SKU: 186000188
Symmetry C18 Column, 300 Å, 3.5 µm, 2.1 mm X 100 mm, 1/pk

Symmetry C18 Column | 186000188


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Product Description

Primarily supports legacy methods. Symmetry columns give you confidence in the long-term compliance of your HPLC methods. No need to compromise on peak shape, selectivity, column-to-column and batch-to-batch reproducibility, or other critical performance characteristics. Available in a variety of particle sizes, chemistries and dimensions.

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Silica

  • pH Range Min

    2 pH

  • pH Range Max

    8 pH

  • Maximum Pressure

    6000 psi (415 Bar)

  • Endcapped

    Yes

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    3.5 µm

  • Endfitting Type

    Waters

  • Pore Size

    300 Å

  • Format

    Column

  • Surface Area

    110

  • System

    HPLC

  • USP Classification

    L1

  • Inner Diameter

    2.1 mm

  • Length

    100 mm

  • Carbon Load

    8.5 %

  • UNSPSC

    41115709

  • Application

    Peptide

  • Brand

    Symmetry

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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Symmetry C18 Column, 300Å, 3.5 µm, 2.1 mm X 100 mm, 1/pk

Symmetry columns continue to set the standard for HPLC drug testing, offering you confidence in your HPLC methods' long-term compliance. There's no need to sacrifice peak shape, selectivity, column-to-column and batch-to-batch consistency, or other essential performance parameters.

You will be able to attain high repeatability in chromatographic findings by adding the Symmetry C18 Column to your equipment. This is made possible by the Symmetry C18 Column's monofunctional bound C18 ligand, which is combined with high purity base-deactivated Silica. The Symmetry C18 Column features one of the industry's tightest ligand surface coverage (ligand density) standards, resulting in minimal variations in resolution and repeatable batch-to-batch results. With the Symmetry C18 Column, you achieve excellent reproducibility in your lab year after year.

Waters created the Symmetry C18 Column with high purity raw materials and column packing techniques, making it one of the most reproducible HPLC columns available. The Symmetry C18 Column comes with a three-page Certificate of Analysis that includes the requirements as well as the results of 22 important tests that each column must pass before being quality certified.

Since the Symmetry C18 Column doesn't need to be revalidated and you won't have to build new procedures for each batch of new columns, you will avoid the labor and lab expenditures that traditionally analytical columns incur due to the ongoing monitoring and QC testing they require. The Symmetry C18 Column is made in specialized Waters facilities that are cGMP, ISO9001, and ISO13485 certified, where each step of the manufacturing process is meticulously monitored and the strictest conditions are maintained. This allows the Symmetry C18 Columns to be manufactured to the strictest specifications in the market, ensuring reliable results every time.

You can shop for lab equipment to meet all of your laboratory needs by browsing our catalog or visiting our website. We also recommend you to review the Symmetry C18 VanGuard Cartridge, 100Å, 3.5 µm, 2.1 mm X 5 mm, 3/pk, which is utilized to extend analytical column lifetime and performance by removing particle contamination from the mobile phase stream.

What Effect Does Temperature Have on Liquid Chromatography?

Chromatography entails a sequence of equilibrium processes wherein analytes can either dissolve in the mobile phase or become adsorbed onto the stationary phase of the column. With an increase in temperature, there is a heightened interchange of analytes between the mobile and stationary phases. Consequently, elevated temperatures expedite the analysis process.