Chemistry |
C4 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
30000 |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Waters |
Pore Size |
300 Å |
Format |
Column |
Surface Area |
110 |
System |
HPLC |
USP Classification |
L26 |
Inner Diameter |
4.6 mm |
Length |
50 mm |
Carbon Load |
2.8 % |
UNSPSC |
41115709 |
Application |
Protein |
Brand |
Symmetry |
Product Type |
Columns |
Units per Package |
1 pk |
Symmetry C4 Column, 300Å, 5 µm, 4.6 mm X 50 mm, 1K - 30K, 1/pk
With the Waters Symmetry C4 Column, which has been intended to set the standard for HPLC drug tests, you may have trust in the long-term compliance of your HPLC procedures. The lab equipment allows critical analysis to be performed without sacrificing peak shape, selectivity, column-to-column and batch-to-batch consistency, or other critical performance parameters. The wide-pored, reversed-phase Symmetry C4 Column is a popular choice in the pharmaceutical business because of its sensitive, robust, and rough HPLC testing. Large peptides and proteins benefit from the lab equipment’s C4 chemistry.
When compared to conventional columns of similar chemistry, the Waters Symmetry C4 Column provides improved peak shape and peak capacity for the separation of biologically active peptides. The Symmetry C4 Column outperforms other columns when it comes to separating hydrophilic and hydrophobic peptides, as well as neutral and highly basic peptides. The Waters Symmetry C4 Column is available in other variations as well. You can shop for lab equipment directly from our website.
The Symmetry C4 Column will perform well in both high and low mass circumstances. For mass spectrometry applications, low TFA mobile phases like 0.1 percent formic acid are advised. Regardless of who uses the method or where it is used, the lab equipment provides the optimum answer for validation compliance.
You get the clearest peaks for biomolecules and higher peak capacity for complicated tryptic maps with the Symmetry C4 Column. This is achieved by the use of ultrapure agents to manufacture silica. As a result, an analytical column with high purity and low silanol activity has been created. The lab equipment is also packed to the best efficiency and reproducibility of any product on the market, providing enhanced precision for impurity and stability tests, more precise quantitation, and adaptation to more demanding techniques.
Protect your analytical column by investing in the Symmetry C4 VanGuard Cartridge, 300Å, 5 µm, 3.9 mm X 5 mm, 1K - 150K, 3/pk. The use of this lab equipment will also extend the analytical column’s lifetime and performance by removing particulate contamination from the mobile phase stream. Since it is made using the exact packing materials as the analytical column, it is well suited to work with it.
What Is The Minimum And Maximum pH Range Offered By The Waters Symmetry C4 Column?
The Symmetry C4 Column listed here offers a wide operating pH of 2-8.