Chemistry |
C4 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
30000 |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Waters |
Pore Size |
300 Å |
Format |
Column |
Surface Area |
110 |
System |
HPLC |
USP Classification |
L26 |
Inner Diameter |
4.6 mm |
Length |
150 mm |
Carbon Load |
2.8 % |
UNSPSC |
41115709 |
Application |
Protein |
Brand |
Symmetry |
Product Type |
Columns |
Units per Package |
1 pk |
Symmetry C4 Column, 300Å, 5 µm, 4.6 mm X 150 mm, 1K - 30K, 1/pk
Get confidence in the long-term compliance of your HPLC methods using the Symmetry C4 Column that has been designed to set the standard for HPLC drug assays. The lab equipment makes it possible to perform critical analysis without any compromise on peak shape, selectivity, column-to-column and batch-to-batch reproducibility, or other important performance characteristics. The sensitive, robust, and rugged HPLC assays offered by the wide-pored, reversed-phase Symmetry C4 Column make it a popular choice in the pharmaceutical industry. Its C4 chemistry is best suited for large peptides and proteins.
Get better peak shape and peak capacity for the separation of biologically active peptides with the Symmetry C4 Column when compared to conventional columns of similar chemistry. When simultaneously separating hydrophilic and hydrophobic peptides, as well as neutral and highly basic peptides, the Symmetry C4 Column exhibits superior performance.
Whether at high or low mass conditions, the Symmetry C4 Column will offer better performance. It is recommended that low TFA mobile phases or 0.1% formic acid be used for mass spectrometry applications. The analytical column offers the best solution for validation compliance, regardless of who uses the method or where.
With the Symmetry C4 Column, you get the sharpest peaks for biomolecules and more peak capacity for complex tryptic maps. This is made possible by the use of the silica that is synthesized using ultrapure agents. The result is an analytical column that is high in purity with a very low silanol activity. The column is also packed to the highest efficiency and reproducibility of any product on the market, giving you improved precision for impurity and stability assays, more accurate quantitation, and adaptability to more rugged methods.
Browse through our brochure or check out our website, through which you easily shop for lab equipment as per the needs of your laboratory.
You may also be interested in Reversed-Phase QC Reference Material; The Reversed-Phase QC Reference Material is a significantly complex reference material mixture in that it contains compounds that could be challenging to separate. It is designed to provide this complexity and is appropriate when complex assays are routinely performed. It can be used with a variety of column chemistries, dimensions, and system hardware.
Why Does My LC System Constantly Shut Down?
HPLC pumps and systems have overpressure protection built in to ensure system safety and protect the columns and equipment. If the system is set too low or there is too much back pressure, the pump may shut off to safeguard the LC system. Cleaning the lines or removing leftover or crystallized material that is clogging or choking the system could be other reasons.