Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
SKU: 186001344 Atlantis dC18 Column, 100Å, 5 µm, 4.6 mm X 150 mm, 1/pk
Atlantis dC18 columns are universal, silica-based, reversed-phase C18 columns that are used for polar compound retention. The Atlantis dC18 columns feature di-functionally bonded C18 ligands that have been optimized for use with highly aqueous mobile phases, including 100% water.
Atlantis dC18 Column, 100Å, 5 µm, 4.6 mm X 150 mm, 1/pk
To achieve the goal of a reversed-phase HPLC column for the retention and separation of polar, water-soluble compounds, Waters knew a new and innovative stationary phase packing material would be necessary to create this unique piece of lab equipment. This led to the creation of the silica-based, difunctionally bonded C18 material used in Atlantis dC18 columns. These columns boast optimized versions of stationary phase physical attributes like end-capping, silica pore size, bonded phase ligand density, and ligand type. These columns, therefore, exhibit superior peak shape, low pH stability, a resistance to dewetting (hydrophobic collapse), and enhanced retention of polar compounds.
The intelligent design of this stationary phase allows the Atlantis dC18 column to exhibit this enhanced retention in polar compounds. Careful design allows for a column that is compatible with the aqueous mobile phase, even with 100% water, as necessary for retaining hydrophilic compounds. This is all done without compromising peak shape for bases due to the fully end-capped nature of the Atlanta dC18 column.
Not only does the Atlantis dC18 Column offer polar compound retention, but all compounds are able to achieve a desirable peak shape while maintaining full compatibility with LC/MS. By combining all the desirable characteristics of the ideal reversed-phase HPLC column, it is suitable for both separating polar compounds in addition to standard reversed-phase applications.
The manufacture of Atlantis columns begins with ultrapure reagents that control the chemical composition and purity of the final product. All columns are manufactured in a cGMP, ISO 9001:200 certified plant, with each step being conducted within narrow tolerances. Further testing can be performed using Reversed-Phase QC Reference Material.
What Solvents Are Appropriate For Atlantis dC18 Columns?
In order to maintain the highest possible column performance, high-quality chromatography grade solvents should always be used. Aqueous buffers require filtering prior to use as well. It is recommended to add at least 5% organic to neutral pH buffers in order to prevent bacterial growth. Pall Corporation Acrodisc filters are recommended by Waters.
Solvents containing suspended particulate materials will likely clog the outside surface of the inlet frit of the column and should be avoided. They can result in higher operating pressure and subsequent poorer performance.
