Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
SKU: 186002012 Atlantis Silica HILIC Column, 100Å, 5 µm, 2.1 mm X 50 mm, 1/pk
Atlantis HILIC Silica columns are used in combination with high organic mobile phases (>80% acetonitrile) to provide retention of analytes that are simply too polar to be retained by reverse-phase chromatography. HILIC separation mechanisms provide orthogonal analyte selectivity compared to traditional chromatographic reversed-phase approaches.
Atlantis Silica HILIC Column, 100Å, 5 µm, 2.1 mm X 50 mm, 1/pk
Easily retaining and separating very polar, water-soluble basic organic compounds has been made possible with the Atlantis Silica HILIC Column. These chemicals include actives, metabolites, and peptides. The Atlantis Silica HILIC Column is able to resolve the challenging separation problems of polar analytes as it is the ideal lab equipment suited to deal with the combination of their hydrophilic nature and net positive charge. This is achieved by using the Hydrophilic Interaction Chromatography (HILIC) alternate separation technique as they are too polar to be retained by reversed-phase HPLC techniques.
Used in combination with high organic mobile phases (>80% acetonitrile), the Atlantis HILIC Silica columns can provide retention of analytes that reverse-phase chromatography finds too polar to be retained. In addition to this, HILIC separation mechanisms provide orthogonal analyte selectivity compared to traditional chromatographic reversed-phase approaches.
With the Atlantis Silica HILIC Column, you also benefit from improved LC/ESI-MS response as well as direct compatibility with SPE solvents and complementary selectivity when compared to the traditional reversed-phase HPLC columns. This means you get results that exhibit higher sensitivity and lower limits of detection. This is especially useful in drug metabolism studies as metabolites are often more polar than their parent compounds and are present in a much lower concentration.
In addition to this, you can leverage the long column lifetime provided by the Atlantis Silica HILIC Column as well as universal compatibility with all LC detectors. The lab equipment offers all this in addition to outstanding column-to-column reproducibility.
Severe peak tailing for polar bases is often observed with the use of the traditional reversed-phase HPLC columns. In contrast, with the Atlantis Silica HILIC Column, you get superior peak shapes for polar bases while leveraging faster and easier method development.
We recommend that you also explore and invest in the Atlantis Silica HILIC VanGuard Cartridge, 100Å, 5 µm, 2.1 mm X 5 mm, 3/pk in order to protect your analytical column from chemical and particulate contaminants. The lab equipment prevents the undesired contaminants from entering the mobile stream and enhances its efficiency as well as its lifetime.
What Is The pH Range Of The Atlantis Silica HILIC Column?
As stated in the provided listing, the Atlantis Silica HILIC Column is specifically designed to operate within a pH range of 1 to 5. This column offers excellent performance and stability under mildly acidic to neutral conditions, making it suitable for a variety of chromatographic applications. Whether working with acidic or slightly alkaline samples, the Atlantis Silica HILIC Column provides reliable and efficient separations, allowing for accurate analysis and characterization of your compounds of interest.
