Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
SKU: 186002015 Atlantis Silica HILIC Column, 100Å, 3 µm, 2.1 mm X 150 mm, 1/pk
Atlantis HILIC Silica columns are used in combination with high organic mobile phases (>80% acetonitrile) to provide retention of analytes that are simply too polar to be retained by reverse-phase chromatography. HILIC separation mechanisms provide orthogonal analyte selectivity compared to traditional chromatographic reversed-phase approaches.
Atlantis Silica HILIC Column, 100Å, 3 µm, 2.1 mm X 150 mm, 1/pk
Use the Atlantis Silica HILIC Column in combination with high organic mobile phases (>80% acetonitrile) to provide retention of analytes that are simply too polar to be retained by reverse-phase chromatography. HILIC (Hydrophilic Interaction Chromatography) separation mechanisms provide orthogonal analyte selectivity compared to traditional chromatographic reversed-phase approaches. The compounds that can be separated by the Atlantis Silica HILIC Column include peptides, metabolites, and actives. In addition to providing retention of very polar compounds, HILIC offers improved LC/ESI-MS response and direct compatibility with SPE solvents. When it is compared to reversed-phase HPLC, the lab equipment also offers complimentary selectivity.
Using reversed-phase chromatography, it is extremely difficult to retain and separate very basic polar analytes, owing to their hydrophilic nature combined with their net positive charge at acidic pH. The use of HILIC resolves these challenging separation problems, which is why the Atlantis Silica HILIC Column is of crucial importance as the ideal lab equipment that can be used in metabolism, drug discovery, and combinatorial chemistries.
The Atlantis Silica HILIC Column enables you to retain highly polar basic analytes with volatile mobile phases that are suitable for compound ionization by ESI-MS. This means you get results that exhibit higher sensitivity and lower limits of detection, which is especially useful in drug metabolism studies as metabolites are often more polar than their parent compounds and are present in a much lower concentration.
Other than offering improved LC/ESI-MS response, the Atlantis Silica HILIC Column also offers direct compatibility with SPE solvents and complementary selectivity when compared to the traditional reversed-phase HPLC columns. In addition to this, in contrast to the traditional reversed-phase HPLC columns where severe peak tailing for polar bases is observed, the use of Atlantis Silica HILIC Column gives superior peak shapes for polar bases while leveraging faster and easier method development.
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The principle of mass spectrometry (MS) is to generate ions from either inorganic or organic compounds by any suitable method, as well as to separate these ions by their mass-to-charge ratio (m/z) and to detect them qualitatively and quantitatively by their respective m/z and abundance.
