SKU: 186002162
BioSuite Diol (OH) Column, 250Å, 4 µm, 4.6 mm X 300 mm, 10K - 500K, 1/pk

BioSuite Diol (OH) Column | 186002162

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Product Description

Primarily supports legacy methods. BioSuite SEC Columns are available in 4.6 mm, 7.5 mm, and 7.8 mm i.d. analytical and 21.5 mm i.d. preparative and guard columns.

Specifications

  • Chemistry

    Diol (OH)

  • Separation Mode

    SEC

  • Particle Substrate

    Silica

  • pH Range Min

    2.5 pH

  • pH Range Max

    7.5 pH

  • Maximum Pressure

    1800 psi (124 bar)

  • Endcapped

    Yes

  • Molecular Weight Range Min

    10000

  • Molecular Weight Range Max

    500000

  • Particle Shape

    Spherical

  • Particle Size

    4 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    250 Å

  • Format

    Column

  • System

    HPLC

  • USP Classification

    L59

  • Inner Diameter

    4.6 mm

  • Length

    300 mm

  • UNSPSC

    41115709

  • Application

    Protein

  • Brand

    BioSuite

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents

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BioSuite Diol (OH) Column, 250Å, 4 µm, 4.6 mm X 300 mm, 10K - 500K, 1/pk

Waters’ BioSuite family of columns is designed to provide high-performance chemistries for those performing isolation, analysis, and characterization of biomolecules in HPLC separations. Designed to meet the demands of challenging protein and peptide analysis, the BioSuite Diol (OH) Column offers maximum separation efficiency. This column uses a wettable, silica-based material that is rigid enough to give chemical stability across a pH range of 2.5 to 7.5. The length and particle size of this column further increases separation efficiency.

The BioSuite Diol (OH) Column’s chemistry makes it the optimal lab equipment for chromatographers performing isolation, analysis, and characterization of proteins and peptides. The column can be used to determine the resolution and recovery of proteins and peptides, yielding reliable results by adequately characterizing complex biomolecules, allowing for the successful development of protein-based biopharmaceuticals and diagnostic reagents. The orthogonal separation techniques of the BioSuite Diol (OH) Column allow for chromatographers to produce next-generation drugs, helping to provide critical characterization data and isolated material required for this task.

Each column is tested against narrow specifications in order to meet industry standards and provide reproducible results. In order to maintain this performance and ensure longer column lifetimes, a BioSuite Diol (OH) Guard Column, 5 µm, 6 mm X 40 mm, 1/pk can be added. This can prevent constant replacements, as it has been designed specifically to work with the BioSuite Diol (OH) Column, offering protection from any mobile phase contamination.

Are Proteins And Peptides The Same Thing?

Peptides and proteins are often referenced together, but they are not the same thing. Both are made up of strings of amino acids and are held together by peptide bonds. However, peptides are made up of smaller chains of amino acids than proteins. The general rule is that a peptide contains two or more amino acids, though the term polypeptides can also refer to a chain of 10 or more amino acids. Protein usually refers to large peptide molecules, which can be either one long chain of 100 or more amino acids, or a composition of several amino acid chains joined together. Hemoglobin would be an example of these larger proteins.